一种参与絮状链霉菌中链黑菌素生物合成的色氨酸C-甲基转移酶。
A tryptophan C-methyltransferase involved in streptonigrin biosynthesis in Streptomyces flocculus.
作者信息
Hartley D L, Speedie M K
出版信息
Biochem J. 1984 May 15;220(1):309-13. doi: 10.1042/bj2200309.
Abstract
A C-methyltransferase that catalyses the transfer of a methyl group from S-adenosylmethionine to C-3 of tryptophan, resulting in beta-methyltryptophan, has been identified in cell-free extracts of streptonigrin-producing Streptomyces flocculus. The absolute configuration of the product was shown to be (2S,3R)-beta-methyltryptophan by high-pressure liquid chromatography and reactivity with D- and L-amino acid oxidases. In shake culture, maximum specific activity occurs after S. flocculus enters stationary phase, but before significant streptonigrin accumulates.
摘要
在产链黑菌素的絮状链霉菌的无细胞提取物中，已鉴定出一种C-甲基转移酶，该酶催化将甲基从S-腺苷甲硫氨酸转移至色氨酸的C-3位，生成β-甲基色氨酸。通过高压液相色谱以及与D-和L-氨基酸氧化酶的反应，证明产物的绝对构型为(2S,3R)-β-甲基色氨酸。在摇瓶培养中，最大比活性在絮状链霉菌进入稳定期后、但在链黑菌素大量积累之前出现。
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