Schultz N A, Benson D R
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-3044.
J Bacteriol. 1989 Dec;171(12):6873-7. doi: 10.1128/jb.171.12.6873-6877.1989.
The ability of nitrogenase-containing Frankia sp. strain CpI1 vesicles to regrow vegetative hyphae is demonstrated. Vesicles attached to hyphae in N2-fixing CpI1 cultures and sucrose gradient-isolated vesicles exhibited hyphal outgrowths when incubated in certain defined liquid media. Single or multiple hyphal extensions grew out from the vesicles.
含固氮酶的弗兰克氏菌属CpI1菌株泡囊再生营养菌丝的能力得到了证实。在固氮的CpI1培养物中附着于菌丝的泡囊以及经蔗糖梯度分离的泡囊,在特定的限定液体培养基中培养时会出现菌丝生长。单个或多个菌丝延伸从泡囊中长出。