Teixeira C, Barbot J, Freitas M I
Department of Pathology, Laboratory Hematology Unit, Centro Hospitalar do Porto, Porto, Portugal.
Department of Medicine, Pediatric Hematology Unit, Centro Hospitalar do Porto, Porto, Portugal.
Int J Lab Hematol. 2015 Oct;37(5):626-30. doi: 10.1111/ijlh.12374. Epub 2015 Apr 28.
New erythrocyte and reticulocyte parameters available on modern hematology analyzers have shown to be useful markers in children. However, pediatric method-specific reference ranges for these indices are sparse. We aim to establish pediatric reference values for reticulocyte parameters and hypochromic red blood cells in healthy children with the ADVIA 2120 hematology analyzer.
Prospective study of 311 healthy children aged from 6 months to 18 years old with normal hematological parameters. ADVIA 2120 hematology analyzer (Siemens Healthcare Diagnostics, Amadora, Portugal) was used for whole-blood hematological measures. The sample population was grouped according to age into three cohorts, and gender distribution was used for participants aged ≥12 years.
Age- and gender-specific reference limits of reticulocyte hemoglobin content (CHr or Ret-He), difference between the reticulocyte and erythrocyte hemoglobin content (Delta-He), immature reticulocyte fraction (IRF) and percentage of hypochromic red blood cells (%Hypo) are provided. Delta-He, IRF and %Hypo showed statistically significant gender differences in the 12-17 years group. CHr presented no significant gender variation within all age groups (median 30.8 ± 1.7 pg).
Establishing reliable pediatric reference intervals for these novel hematological parameters may offer valuable prospects for clinical practice and research in the pediatrics field.
现代血液分析仪提供的新红细胞和网织红细胞参数已被证明是儿童有用的标志物。然而，这些指标的儿科特定方法参考范围稀少。我们旨在通过ADVIA 2120血液分析仪建立健康儿童网织红细胞参数和低色素红细胞的儿科参考值。
对311名6个月至18岁血液学参数正常的健康儿童进行前瞻性研究。使用ADVIA 2120血液分析仪（西门子医疗诊断公司，葡萄牙阿马多拉）进行全血血液学检测。样本人群按年龄分为三个队列，≥12岁的参与者按性别分布。
提供了网织红细胞血红蛋白含量（CHr或Ret-He）、网织红细胞与红细胞血红蛋白含量之差（Delta-He）、未成熟网织红细胞比例（IRF）和低色素红细胞百分比（%Hypo）的年龄和性别特异性参考限值。Delta-He、IRF和%Hypo在12 - 17岁组中显示出统计学上显著的性别差异。CHr在所有年龄组中均无显著性别差异（中位数30.8 ± 1.7 pg）。
为这些新的血液学参数建立可靠的儿科参考区间可能为儿科领域的临床实践和研究提供有价值的前景。
