Soleimani Afsane, Asadi Jahanbakhsh, Rostami-Charati Faramarz, Gharaei Roghaye
Departments of Chemistry, Faculty of Science, Gonbad Kavous University, P.O.Box 163, Gonbad, Iran.
Comb Chem High Throughput Screen. 2015;18(5):505-13. doi: 10.2174/1386207318666150430114815.
This study was focused on evaluation of the cytotoxicity and apoptotic affects of benzofuran derivative on MCF-7 breast cancer cell line. This effective compound was isolated from the root of Petasites hybridus plant. For experiments, the MCF-7 cells were treated with several concentrations (0-500μM) of 1-(6-hydroxy-2- isopropenyl-1-benzofuran-5-yl)-1-ethanone 1 at different times. In this study, test of neutral red was also employed for cytotoxicity assay and quantity of P53, P21. Bax genes expression was analyzed using Real-Time PCR and ELISA techniques. Results show that compound 1 has cytotoxicity and apoptotic effects on Human breast cancer (Michigan Cancer Foundation-7) MCF-7 cells.
本研究聚焦于评估苯并呋喃衍生物对MCF-7乳腺癌细胞系的细胞毒性和凋亡影响。这种有效化合物是从蜂斗菜属植物的根部分离得到的。实验中，MCF-7细胞在不同时间用几种浓度（0 - 500μM）的1-(6-羟基-2-异丙烯基-1-苯并呋喃-5-基)-1-乙酮1进行处理。在本研究中，中性红试验也用于细胞毒性测定以及P53、P21的定量分析。采用实时荧光定量PCR和酶联免疫吸附测定技术分析Bax基因的表达。结果表明，化合物1对人乳腺癌（密歇根癌症基金会-7）MCF-7细胞具有细胞毒性和凋亡作用。
