Saum Kai-Uwe, Dieffenbach Aida Karina, Jansen Eugène H J M, Schöttker Ben, Holleczek Bernd, Hauer Klaus, Brenner Hermann
Division of Clinical Epidemiology and Aging Research, German Cancer Research Center (DKFZ), Heidelberg, Germany.
Gerontology. 2015;61(5):407-15. doi: 10.1159/000380881. Epub 2015 Apr 24.
Oxidative stress (OS) and inflammatory biomarkers have been postulated to be important factors in the development of age-related diseases. While causes of frailty are complex and multidimensional based on the interaction of genetic, biological, physical, and environmental factors, the biological basis of frailty has been difficult to establish.
In this study, we aimed to assess the possible association between different OS and inflammatory biomarkers and frailty.
This cross-sectional analysis was performed among 2,518 subjects participating in a large population-based cohort study on aging conducted in Germany. Frailty was assessed as proposed by Fried et al. [J Gerontol A Biol Sci Med Sci 2001;56:M146-M156]. OS biomarkers, biological antioxidant potential (BAP), derivate of reactive oxygen metabolites (d-ROM) and total thiol levels (TTL), and an established biomarker of inflammation C-reactive protein (CRP) were measured by spectrophotometry and immunoturbidimetry. Logistic regression models were performed to assess the relationship between the OS biomarkers and frailty status. Odds ratios (OR) and 95% confidence intervals (CI) were calculated to quantify the associations.
Mean levels of d-ROM, TTL, and CRP differed between frail and non-frail participants (p values <0.0001). Comparing highest and lowest quartiles of the biomarkers, statistically significant positive associations with frailty were observed for d-ROM (OR: 2.02, 95% CI: 1.25-3.25) and CRP (OR: 3.15, 95% CI: 2.00-4.96), respectively, after controlling for age and sex. An inverse statistically significant association with frailty was observed for TTL (OR: 0.42, 95% CI: 0.25-0.69).
The strong associations with OS biomarkers and CRP support a major role of OS and inflammation in the development of frailty, which should be followed up in further longitudinal studies on frailty.
氧化应激（OS）和炎症生物标志物被认为是与年龄相关疾病发生发展的重要因素。虽然基于遗传、生物、物理和环境因素的相互作用，衰弱的原因复杂且具有多维度性，但衰弱的生物学基础一直难以确立。
在本研究中，我们旨在评估不同的OS和炎症生物标志物与衰弱之间可能存在的关联。
本横断面分析在参与德国一项基于大规模人群的衰老队列研究的2518名受试者中进行。按照Fried等人[《老年学杂志A：生物科学与医学科学》2001年；56：M146 - M156]提出的方法评估衰弱情况。通过分光光度法和免疫比浊法测量OS生物标志物、生物抗氧化潜能（BAP）、活性氧代谢产物衍生物（d-ROM）和总巯基水平（TTL），以及一种已确立的炎症生物标志物C反应蛋白（CRP）。采用逻辑回归模型评估OS生物标志物与衰弱状态之间的关系。计算比值比（OR）和95%置信区间（CI）以量化关联。
衰弱和非衰弱参与者之间d-ROM、TTL和CRP的平均水平存在差异（p值<0.0001）。在对年龄和性别进行校正后，比较生物标志物的最高和最低四分位数，发现d-ROM（OR：2.02，95% CI：1.25 - 3.25）和CRP（OR：3.15，95% CI：2.00 - 4.96）分别与衰弱存在统计学显著的正相关。观察到TTL与衰弱存在统计学显著的负相关（OR：0.42，95% CI：0.25 - 0.69）。
与OS生物标志物和CRP的强关联支持了OS和炎症在衰弱发生发展中的重要作用，这一点应在关于衰弱的进一步纵向研究中进行追踪。
