Gel permeation chromatography combined with capillary electrophoresis for microanalysis of proteins.

A new type of apparatus for the analysis of complex protein mixtures, in which gel permeation chromatography was combined with capillary electrophoresis, was constructed. Proteins were separated according to their molecular size in the first step, then separated according to their electrophoretic mobility in the second step. The outlet of a microbore column was connected with the sample injection port of a capillary electrophoresis apparatus. All the procedures of chromatography and electrophoresis were automated with the aid of a system controller. Preliminary results on the separation of proteins are presented.