Territo M C, Ganz T, Selsted M E, Lehrer R
Department of Medicine, University of California, Los Angeles 90024.
J Clin Invest. 1989 Dec;84(6):2017-20. doi: 10.1172/JCI114394.
We investigated the monocyte-chemotactic activity of fractionated extracts of human neutrophil granules. Monocyte-chemotactic activity was found predominantly in the defensin-containing fraction of the neutrophil granules. Purified preparations of each of the three human defensins (HNP-1, HNP-2, HNP-3) were then tested. HNP-1 demonstrated significant chemotactic activity for monocytes: Peak activity was seen at HNP-1 concentrations of 5 X 10(-9) M and was 49 +/- 20% (mean +/- SE, n = 9) of that elicited by 10(-8) M FMLP. HNP-2 (peak activity at 5 X 10(-9) M) was somewhat less active, yielding 19 +/- 10% (n = 11). HNP-3 failed to demonstrate chemotactic activity. Checkerboard analysis of monocyte response to HNP-1 and HNP-2 confirmed that their activity was chemotactic rather than chemokinetic. Neutrophils demonstrated a low level of response to defensins but this reaction was primarily chemokinetic. Defensins may play a role in the recruitment of monocytes by neutrophils into inflammatory sites.
我们研究了人中性粒细胞颗粒分级提取物的单核细胞趋化活性。单核细胞趋化活性主要存在于中性粒细胞颗粒中含防御素的部分。然后对三种人防御素（HNP-1、HNP-2、HNP-3）的纯化制剂进行了测试。HNP-1对单核细胞表现出显著的趋化活性：在HNP-1浓度为5×10⁻⁹ M时出现峰值活性，为10⁻⁸ M FMLP诱导活性的49±20%（平均值±标准误，n = 9）。HNP-2（在5×10⁻⁹ M时出现峰值活性）活性稍低，为19±10%（n = 11）。HNP-3未表现出趋化活性。对单核细胞对HNP-1和HNP-2反应的棋盘分析证实，它们的活性是趋化性的而非化学动力学性的。中性粒细胞对防御素的反应水平较低，但这种反应主要是化学动力学性的。防御素可能在中性粒细胞将单核细胞募集到炎症部位中发挥作用。
