Rhoades R W, Fish S E, Chiaia N L, Bennett-Clarke C, Mooney R D
Department of Anatomy, Medical College of Ohio, Toledo 43699-0008.
J Comp Neurol. 1989 Nov 22;289(4):641-56. doi: 10.1002/cne.902890409.
Anterograde tracing with Phaseolus vulgaris leucoagglutinin (PHA-L) and intra-axonal recording and injection techniques were employed to describe the projection from the trigeminal (V) brainstem complex to the deep laminae of the superior colliculus (SC) in the hamster and the rat. The organization of these projections was the same in the two species. Deposits of PHA-L into V nucleus principalis (PrV) produced labelled axons and boutonlike swellings in the lower stratum griseum intermediale (SGI) and upper stratum album intermedium (SAI) in the SC bilaterally. Plots of boutonlike swellings indicated that the terminals of this projection were arrayed in clusters. Nucleus principalis also projected to the stratum griseum profundum (SGP) and stratum album profundum (SAP). This deeper projection did not terminate in clusters and it was most prominent in the lateral SC. The ipsilateral PrV-SC projection appeared to arise mainly from axons that recrossed the midline at the level of the SC commissure. Reconstruction of individual PHA-L labelled fibers demonstrated that single axons gave rise to terminals on both sides of the midline. Deposits of PHA-L into V subnucleus interpolaris (SpI) yielded results that were identical to those obtained with PrV injections with one exception: none of these deposits produced any labelled terminals in the ipsilateral SC. Deposits of PHA-L into V subnucleus caudalis (SpC) produced only sparse labelling in SC. Most labelled swellings were located in the SGP and SAP and they were visible only in the SC contralateral to the PHA-L injection site. Single axons arising from cells in SpI were recorded and injected with horseradish peroxidase (HRP) in the hamster's SC. These fibers all responded to stimulation of multiple mystacial vibrissae and gave rise to 2-5 clusters of bouton-like swellings in the lower SGI and upper SAI.
采用菜豆白细胞凝集素（PHA-L）顺行示踪以及轴突内记录和注射技术，来描述仓鼠和大鼠三叉神经（V）脑干复合体至上丘（SC）深层的投射。这两个物种中这些投射的组织方式相同。将PHA-L注入三叉神经主核（PrV），在双侧SC的中间灰质下层（SGI）和中间白质上层（SAI）产生了标记轴突和纽扣状膨大。纽扣状膨大的分布图表明，该投射的终末呈簇状排列。主核也投射到深层灰质（SGP）和深层白质（SAP）。这种更深层的投射并不呈簇状终末，且在SC外侧最为明显。同侧PrV-SC投射似乎主要源于在SC连合水平重新交叉中线的轴突。对单个PHA-L标记纤维的重建表明，单根轴突在中线两侧都有终末。将PHA-L注入三叉神经极间核（SpI）得到的结果与注入PrV的结果相同，只有一个例外：这些注入均未在同侧SC产生任何标记终末。将PHA-L注入三叉神经尾侧核（SpC）在SC中仅产生稀疏标记。大多数标记膨大位于SGP和SAP，且仅在与PHA-L注射部位对侧的SC中可见。在仓鼠的SC中记录并向源于SpI细胞的单根轴突注射辣根过氧化物酶（HRP）。这些纤维均对多个触须的刺激产生反应，并在SGI下层和SAI上层产生2 - 5簇纽扣状膨大。
