Pérez-Clausell J, Frederickson C J, Danscher G
Anatomisk Institut B, Aarhus Universitet, Denmark.
J Comp Neurol. 1989 Dec 8;290(2):201-12. doi: 10.1002/cne.902900204.
Many regions of the basal forebrain are innervated by zinc-containing axonal boutons. In the present work, the lesion/degeneration method, coupled with histochemical staining for zinc-containing boutons, was used to determine the origins and efferent pathways of these zinc-containing projections to the basal forebrain. Knife cuts of the stria terminalis or extensive electrolytic lesions of the amygdala resulted in the bleaching of the staining for zinc (Timm stain) and terminal degeneration (Fink-Heimer method) ipsilaterally in the following areas: granule cell layer of the accessory olfactory bulb, shell of nucleus accumbens, bed nucleus of the stria terminalis, striohypothalamic nucleus, retrochiasmatic area, ventromedial hypothalamic nucleus (in the cell-sparse shell), medial tuberal nucleus, terete hypothalamic nucleus, and ventral premammillary nucleus. Small lesions made with ibotenic acid in the posteromedial part of the amygdalohippocampal area caused bleaching of the stain for zinc in the accessory olfactory bulb, in the medial zone of the bed nucleus of the stria terminalis, and in the ventral premammillary nucleus. Lesions in either the ventral subiculum or the anterolateral part of the amygdalohippocampal area caused bleaching in the ventromedial hypothalamic nucleus. Lesions in the hippocampus or in the neocortex did not produce bleaching of the stain for zinc in the above-mentioned terminal fields. The present results agree with previous studies on amygdaloid efferents and suggest that neurons in the amygdalohippocampal area and, possibly, in the ventral subiculum give origin to zinc-containing boutons.
基底前脑的许多区域都由含锌的轴突终末支配。在本研究中，采用损伤/变性方法，结合含锌终末的组织化学染色，来确定这些含锌投射至基底前脑的起源和传出通路。切断终纹或广泛电解杏仁核，会导致同侧以下区域的锌染色（Timm染色）脱色和终末变性（Fink-Heimer法）：副嗅球颗粒细胞层、伏隔核壳、终纹床核、纹状体下丘脑核、视交叉后区、下丘脑腹内侧核（细胞稀疏的壳区）、内侧结节核、圆柱形下丘脑核和乳头体前腹侧核。用鹅膏蕈氨酸在杏仁海马区后内侧部分造成的小损伤，会导致副嗅球、终纹床核内侧区和乳头体前腹侧核的锌染色脱色。海马或新皮质的损伤不会导致上述终末区域的锌染色脱色。目前的结果与先前关于杏仁核传出纤维的研究一致，并表明杏仁海马区以及可能的腹侧下托中的神经元发出含锌终末。
