Jenkins K J
Animal Research Centre, Agriculture Canada, Ottawa, Ontario.
J Dairy Sci. 1989 Sep;72(9):2346-50. doi: 10.3168/jds.S0022-0302(89)79367-X.
The subcellular distribution of Cu, Zn, Fe, and Mo was investigated in liver homogenates from preruminant calves fed control Cu (10 ppm), high Cu (1000 ppm), or high Cu plus high Zn (1000 ppm) milk replacer. For controls, Cu was located primarily in the nuclei and large granule fractions, Zn mainly in the cytosol, and Fe in the cytosol and nuclei fraction; Mo was present in all compartments but least in microsomes. Calves fed high Cu had markedly increased hepatic Cu concentration in the nuclei and cytosol fractions, reduced cytosol Zn, increased nuclei Fe, and decreased Mo concentration in all cell compartments. Feeding high Zn with high Cu (which prevented deaths from high Cu) reversed some changes in hepatic trace element patterns caused by high Cu while initiating new alterations. The marked increase in hepatic Cu and reduced Mo in nuclei and cytosol after Cu loading indicate that these compartments may have a predominant role in the development of Cu toxicity in the preruminant calf.
研究了给反刍前犊牛饲喂对照铜（10 ppm）、高铜（1000 ppm）或高铜加高锌（1000 ppm）代乳粉后，肝脏匀浆中铜、锌、铁和钼的亚细胞分布。对于对照组，铜主要位于细胞核和大颗粒部分，锌主要在胞质溶胶中，铁在胞质溶胶和细胞核部分；钼存在于所有区室中，但在微粒体中含量最少。饲喂高铜的犊牛细胞核和胞质溶胶部分的肝脏铜浓度显著增加，胞质溶胶锌减少，细胞核铁增加，所有细胞区室中的钼浓度降低。高锌与高铜一起饲喂（这可防止因高铜导致的死亡）逆转了高铜引起的肝脏微量元素模式的一些变化，同时引发了新的改变。铜负荷后细胞核和胞质溶胶中肝脏铜的显著增加以及钼的减少表明，这些区室可能在反刍前犊牛铜中毒的发展中起主要作用。
