Kikuchi Y, Oomori K, Miyauchi M, Kita T, Kuki E, Iwano I, Hirata J, Nagata I
Department of Obstetrics and Gynecology, National Defense Medical College, Saitama.
Nihon Sanka Fujinka Gakkai Zasshi. 1989 Dec;41(12):1891-5.
Effect of intraperitoneal instillations of interleukin-2 (IL-2) and/or lymphokine-activated killer (LAK) cells on the ascites formation and the survival time was examined by using a nude mice model with malignant ascites by intraperitoneal inoculation of human ovarian cancer cells (HRA) derived from ascites of a patient with serous cystadenocarcinoma of the ovary. On 28 days after tumor inoculation, all nude mice in both untreated and spleen cells only treated groups formed ascites. Two of 10 nude mice treated with IL-2 only after tumor inoculation survived without forming ascites during the experimental period. On the other hand, all nude mice treated with LAK cells only formed ascites by 14 days after tumor inoculation. When LAK cells and IL-2 were combined 5 of 10 mice survived without forming ascites during the experimental period. The survival time of the IL-2 only treated group was significantly prolonged, compared to that of medium only, spleen cells only and LAK cells only treated groups. When administration of LAK cells was followed by IL-2, the survival time was further prolonged.
通过使用裸鼠模型，腹腔接种源自卵巢浆液性囊腺癌患者腹水的人卵巢癌细胞（HRA）诱导恶性腹水，研究腹腔内注入白细胞介素-2（IL-2）和/或淋巴因子激活的杀伤细胞（LAK）对腹水形成和存活时间的影响。肿瘤接种后28天，未治疗组和仅用脾细胞治疗组的所有裸鼠均形成腹水。肿瘤接种后仅用IL-2治疗的10只裸鼠中有2只在实验期间存活且未形成腹水。另一方面，仅用LAK细胞治疗的所有裸鼠在肿瘤接种后14天形成腹水。当LAK细胞和IL-2联合使用时，10只小鼠中有5只在实验期间存活且未形成腹水。与仅用培养基、仅用脾细胞和仅用LAK细胞治疗组相比，仅用IL-2治疗组的存活时间显著延长。当先给予LAK细胞后给予IL-2时，存活时间进一步延长。
