Song Y F, Zhao Y N, Li J
Dong Fang Hospital, Fuzhou.
Zhonghua Fu Chan Ke Za Zhi. 1994 Jun;29(6):332-5, 381.
The study of generation of LAK cells from cord blood following a brief exposure to interleukin-2 (IL-2) demonstrated that a short time induction (15min-1h) of lymphocytes by a high concentration of IL-2 resulted in the maturation of LAK proceusor cells and such activated cells expressed cytolytic activity comparable to that of routinely induced (cultured continuously in IL-2 for 3-7 days) lymphocytes. Serologic test showed that this efficiency was not inhibited by autoserum. The results of experimental therapy with the two kinds of LAK cells in nude mouse model of human ovarian cancer confirmed that both types of LAK cells had remarkable inhibitory effect on tumor growth. The preliminary trial in the treatment of advanced ovarian cancer with rapid-pulsed LAK cells suggested that this method may serve as a new approach in the clinical application of tumor immunotherapy.
对脐带血淋巴细胞经短暂暴露于白细胞介素-2（IL-2）后生成LAK细胞的研究表明，高浓度IL-2对淋巴细胞进行短时间诱导（15分钟至1小时）可导致LAK前体细胞成熟，且此类活化细胞表现出的细胞溶解活性与常规诱导（在IL-2中连续培养3至7天）的淋巴细胞相当。血清学检测表明，这种效应不受自身血清的抑制。在人卵巢癌裸鼠模型中对这两种LAK细胞进行实验性治疗的结果证实，两种类型的LAK细胞对肿瘤生长均有显著抑制作用。用快速脉冲LAK细胞治疗晚期卵巢癌的初步试验表明，该方法可能成为肿瘤免疫治疗临床应用的一种新途径。
