[乙型流感病毒维多利亚系血凝素分子的表位分析]

[Epitope analysis of the hemagglutinin molecule of the Victoria lineage influenza B viruses].

作者信息

Sorokin E V, Tsareva T R, Sominina A A, Pisareva M M, Komissarov A V, Kosheleva A A, Grudinin M P

出版信息

Vopr Virusol. 2014;59(6):27-31.

Abstract

A panel of five monoclonal antibodies (MAbs) to the HA1 molecule of the influenza B virus of the Victorian lineage with high virus-neutralizing activity was developed. For identification of the virus neutralizing epitopes in HA1 escape mutants (EM) of the influenza BIShandong/07/97 and B/Malaysia/2506/04 virus were selected using virus- neutralizing antibodies (MAbs). Three EMs had single, two--double and one--triple amino acid substitutions (AAS) in HA1 (H122N, A202E, K203T, K2031, K203N or A317V). In addition, AAS N197S was detected in three EMs. A correlation of AAS identified with peculiarities of interaction of EMs with Mabs was discussed.

摘要

研制出一组针对维多利亚谱系乙型流感病毒HA1分子的五种具有高病毒中和活性的单克隆抗体（MAb）。为了鉴定乙型流感病毒山东/07/97和B/马来西亚/2506/04病毒HA1逃逸突变体（EM）中的病毒中和表位，使用病毒中和抗体（MAb）进行筛选。三个EM在HA1中有单个、两个或三个氨基酸替换（AAS）（H122N、A202E、K203T、K203I、K203N或A317V）。此外，在三个EM中检测到AAS N197S。讨论了所鉴定的AAS与EM与单克隆抗体相互作用特性之间的相关性。