Cui Guanglin, Zhu Runan, Deng Jie, Zhao Linqing, Sun Yu, Wang Fang, Qian Yuan
Laboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, 2 Yabao Road, Beijing 100020, China.
Laboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, 2 Yabao Road, Beijing 100020, China.
Infect Genet Evol. 2015 Jul;33:163-8. doi: 10.1016/j.meegid.2015.04.025. Epub 2015 Apr 27.
Human respiratory syncytial virus (HRSV) is the most common viral pathogen causing lower respiratory infections in infants and young children worldwide. HRSV ON1 genotype in subgroup A with a characteristic of a 72 nucleotide duplication in the second highly variable region of attachment glycoprotein gene, has been reported in some countries since it was first detected in clinical samples collected in Canada in 2010. In this study, 557 HRSV antigen-positive nasopharyngeal aspirates were randomly selected during 2012/2013 to 2013/2014 HRSV seasons in Beijing for subgroup typing and for ON1 genotype screening by using a PCR based method developed for easily identifying genotype ON1 out of strains of subtype A. It was found that subgroup B was dominant in the 2012/2013 season and sudden shift of subgroup dominance from B to A and rapid replacement of previously prevailing NA1 genotype by ON1 genotype occurred in the 2013/2014 season. Reversible amino acid replacement in the G protein gene was found in a new branch of ON1 genotype. The evolutionary rate of the 351 global ON1 sequences was estimated to 7.34 × 10(-3) nucleotide substitutions per site per year (95% highest probability density intervals, HPD, 5.71 × 10(-3) to 9.04 × 10(-3)), with the time of most recent common ancestor dating back to June 2009.
人呼吸道合胞病毒（HRSV）是全球范围内引起婴幼儿下呼吸道感染的最常见病毒病原体。A亚组中的HRSV ON1基因型在附着糖蛋白基因的第二个高变区具有72个核苷酸重复的特征，自2010年在加拿大收集的临床样本中首次检测到以来，已在一些国家被报道。在本研究中，于2012/2013至2013/2014年HRSV流行季期间在北京随机选取了557份HRSV抗原阳性的鼻咽抽吸物进行亚组分型，并使用一种基于PCR的方法进行ON1基因型筛查，该方法便于从A亚型毒株中鉴定出基因型ON1。结果发现，B亚组在2012/2013流行季占主导地位，而在2013/2014流行季出现了亚组优势从B向A的突然转变，并且ON1基因型迅速取代了先前流行的NA1基因型。在ON1基因型的一个新分支中发现了G蛋白基因中的可逆氨基酸替换。估计351条全球ON1序列的进化速率为每年每个位点7.34×10⁻³个核苷酸替换（95%最高概率密度区间，HPD，5.71×10⁻³至9.04×10⁻³），最近共同祖先的时间可追溯到2009年6月。
