[2009年至2014年中国浙江省南部呼吸道合胞病毒分子流行病学研究]
[A molecular epidemiological study of respiratory syncytial virus circulating in southern Zhejiang Province, China, from 2009 to 2014].
作者信息
Dong Yi-Wei, Dai Li-Hong, Ye Wen-Jing, Chen Xiao-Fang, Dong Lin
机构信息
Department of Pulmonology, Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2018 Nov;20(11):904-910. doi: 10.7499/j.issn.1008-8830.2018.11.005.
OBJECTIVE
To find out the prevalence of respiratory syncytial virus (RSV) genotypes in southern Zhejiang Province, China, and to study the genetic characteristics of G protein from subtype A of RSV.
METHODS
The lower respiratory tract secretions of children under 5 years of age who were hospitalized for pneumonia and bronchiolitis in three hospitals in southern Zhejiang Province from July 2009 to June 2014 were collected. Direct immunofluorescence assay was used to detect RSV antigens from the collected secretions. A total of 200 samples were randomly selected from RSV-positive specimens in each prevailing year (from July of a specific year to June of the next year). RT-PCR was used to determine RSV subtypes, and the near-full length gene sequence of G protein from subtype A was amplified and sequenced to identify the genotype.
RESULTS
A total of 25 449 samples of lower respiratory tract secretions were collected from 2009 to 2014, among which 6 416 (25.21%) samples were RSV-positive. Among the 1 000 RSV-positive specimens randomly sampled, 462 strains (46.2%) were subtype A, and 538 strains (53.8%) were subtype B. Subtype A accounted for 22.5%, 74.5%, 84.5%, 19.0%, and 30.5% of the total strains in each year from 2009 to 2014. A total of 25 RSV subtype A strains were randomly sampled and sent out for bidirectional sequencing in each year, which confirmed 52 positive subtype A strains. Four genotypes of subtype A strains were obtained from the above strains, including NA1 (39 strains), NA4 (1 strain), ON1 (10 strains), and GA2 (2 strains). NA1 was the dominant genotype between 2009 and 2012, and ON1 was the only genotype of subtype A during 2013-2014. The nucleotide homology and amino acid homology between the G protein of subtype A and the prototype strain A2 were 80.7%-89.3% and 74.4%-82.6%, respectively. The nucleotide homology and amino acid homology between the isolates of subtype A were 81.5%-100% and 80.2%-100%, respectively.
CONCLUSIONS
In southern Zhejiang Province from 2009 to 2014, there was a co-circulation of RSV subtypes A and B, as well as a co-circulation of several different genotypes of RSV subtype A, which had highly variable G protein genes.
目的
了解中国浙江省南部呼吸道合胞病毒（RSV）基因型的流行情况，并研究RSV A亚型G蛋白的遗传特征。
方法
收集2009年7月至2014年6月期间在浙江省南部三家医院因肺炎和支气管炎住院的5岁以下儿童的下呼吸道分泌物。采用直接免疫荧光法检测所收集分泌物中的RSV抗原。在每个流行年份（从某一年的7月至次年的6月）从RSV阳性标本中随机选取200份样本。采用逆转录聚合酶链反应（RT-PCR）确定RSV亚型，并扩增和测序RSV A亚型G蛋白的近全长基因序列以鉴定基因型。
结果
2009年至2014年共收集25449份下呼吸道分泌物样本，其中6416份（25.21%）样本RSV呈阳性。在随机抽取的1000份RSV阳性标本中，462株（46.2%）为A亚型，538株（53.8%）为B亚型。2009年至2014年各年份A亚型分别占总毒株的22.5%、74.5%、84.5%、19.0%和30.5%。每年随机抽取25株RSV A亚型毒株进行双向测序，共确认52株A亚型阳性毒株。从上述毒株中获得了A亚型毒株的四种基因型，包括NA1（39株）、NA4（1株）、ON1（10株）和GA2（2株）。NA1是2009年至2012年期间的优势基因型，ON1是2013 - 2014年期间A亚型的唯一基因型。A亚型G蛋白与原型株A2的核苷酸同源性和氨基酸同源性分别为80.7% - 89.3%和74.4% - 82.6%。A亚型分离株之间的核苷酸同源性和氨基酸同源性分别为81.5% - 100%和80.2% - 100%。
结论
2009年至2014年在浙江省南部，RSV A、B亚型共同流行，且RSV A亚型的几种不同基因型也共同流行，其G蛋白基因具有高度变异性。
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