Zhang Jing, Dong Jian, Yang Zuozhang, Ma Xiang, Zhang Jinlei, Li Mei, Chen Yun, Ding Yingying, Li Kun, Zhang Zhiping
Bone and Soft Tissue Tumors Research Center of Yunnan Province, Department of Orthopaedics, The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming, Yunnan, 650118, People's Republic of China.
Department of Pathology, The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming, Yunnan, 650118, People's Republic of China.
World J Surg Oncol. 2015 May 1;13:168. doi: 10.1186/s12957-015-0579-5.
This research aimed to study the role of ezrin, CD44, and VEGF in invasion, metastasis, recurrence, and prognosis of giant cell tumor of bone (GCTB) and its association with the clinical and pathological features of GCTB.
Expression status of ezrin, CD44, and VEGF in 80 GCTB tissues and its adjacent noncancerous tissue samples were measured with immunohistochemical and Elivison staining. Their correlation with the clinical and pathologic factors was statistically analyzed by chi-square test.
The expression status of ezrin, CD44, and VEGF were significantly higher in GCTB tissue samples than in its adjacent noncancerous tissue samples and in GCTB at Campanacci stage III than in Campanacci stages I and II (P < 0.05). No significant difference was found in age and sex of the patients and locations of the tumor (P > 0.05). Survival analysis showed that the expression status of ezrin, CD44, VEGF, and Campanacci clinical stages of GCTB were positively associated with the survival rate of GCTB patients and negatively associated with ezrin and Campanacci stages of GCTB, indicating that ezrin, CD44, VEGF, and Campanacci clinical stages of GCTB are the independent factors for GCTB.
Ezrin, CD44, and VEGF are over-expressed in GCTB tissue and its adjacent noncancerous tissue samples and may play an important role in the occurrence, invasion, metastasis, and recurrence of GCTB. Measurement of ezrin, CD44, and VEGF expression status may contribute to the judgment of prognosis of GCTB patients.
本研究旨在探讨埃兹蛋白（ezrin）、CD44和血管内皮生长因子（VEGF）在骨巨细胞瘤（GCTB）侵袭、转移、复发及预后中的作用及其与GCTB临床病理特征的关系。
采用免疫组织化学和Elivison染色法检测80例GCTB组织及其癌旁组织样本中ezrin、CD44和VEGF的表达情况。采用卡方检验对其与临床病理因素的相关性进行统计学分析。
GCTB组织样本中ezrin、CD44和VEGF的表达水平显著高于其癌旁组织样本，且Campanacci III期GCTB中的表达水平高于Campanacci I期和II期（P < 0.05）。患者的年龄、性别及肿瘤部位差异无统计学意义（P > 0.05）。生存分析显示，GCTB患者的ezrin、CD44、VEGF表达水平及Campanacci临床分期与生存率呈正相关，与ezrin和Campanacci分期呈负相关，表明GCTB的ezrin、CD44、VEGF及Campanacci临床分期是影响GCTB患者生存的独立因素。
Ezrin、CD44和VEGF在GCTB组织及其癌旁组织样本中高表达，可能在GCTB的发生、侵袭、转移及复发中起重要作用。检测ezrin、CD44和VEGF的表达水平可能有助于判断GCTB患者的预后。
