Hollingdale Christie, Thomas Krista, Lewis Nancy, Békri Khalida, McCarron Pearse, Quilliam Michael A
Measurement Science and Standards, Biotoxin Metrology, National Research Council of Canada, 1411 Oxford Street, Halifax, NS, B3H 3Z1, Canada.
Anal Bioanal Chem. 2015 Jul;407(18):5353-63. doi: 10.1007/s00216-015-8695-1. Epub 2015 May 1.
The worldwide increase in cyanobacterial contamination of freshwater lakes and rivers is of great concern as many cyanobacteria produce potent hepatotoxins and neurotoxins (cyanotoxins). Such toxins pose a threat to aquatic ecosystems, livestock, and drinking water supplies. In addition, dietary supplements prepared from cyanobacteria can pose a risk to consumers if they contain toxins. Analytical monitoring for toxins in the environment and in consumer products is essential for the protection of public health. Reference materials (RMs) are an essential tool for the development and validation of analytical methods and are necessary for ongoing quality control of monitoring operations. Since the availability of appropriate RMs for cyanotoxins has been very limited, the present study was undertaken to examine the feasibility of producing a cyanobacterial matrix RM containing various cyanotoxins. The first step was large-scale culturing of various cyanobacterial cultures that produce anatoxins, microcystins, and cylindrospermopsins. After harvesting, the biomass was lyophilized, blended, homogenized, milled, and bottled. The moisture content and physical characteristics were assessed in order to evaluate the effectiveness of the production process. Toxin levels were measured by liquid chromatography with tandem mass spectrometry and ultraviolet detection. The reference material was found to be homogeneous for toxin content. Stability studies showed no significant degradation of target toxins over a period of 310 days at temperatures up to +40 °C except for the anatoxin-a, which showed some degradation at +40 °C. These results show that a fit-for-purpose matrix RM for cyanotoxins can be prepared using the processes and techniques applied in this work.
全球范围内淡水湖泊和河流中蓝藻污染的增加令人高度关注，因为许多蓝藻会产生强效肝毒素和神经毒素（蓝藻毒素）。此类毒素对水生生态系统、牲畜和饮用水供应构成威胁。此外，由蓝藻制备的膳食补充剂若含有毒素，会对消费者构成风险。对环境和消费品中的毒素进行分析监测对于保护公众健康至关重要。参考物质是分析方法开发和验证的重要工具，也是监测操作持续质量控制所必需的。由于适用于蓝藻毒素的参考物质非常有限，本研究旨在探讨生产一种含有多种蓝藻毒素的蓝藻基质参考物质的可行性。第一步是大规模培养产生类毒素、微囊藻毒素和柱孢藻毒素的各种蓝藻培养物。收获后，将生物质冻干、混合、均质化、研磨并装瓶。评估了水分含量和物理特性，以评估生产过程的有效性。通过液相色谱 - 串联质谱法和紫外检测法测量毒素水平。发现该参考物质的毒素含量均匀。稳定性研究表明，除了在 +40°C 时类毒素 -a 有一定降解外，在高达 +40°C 的温度下，目标毒素在 310 天内没有显著降解。这些结果表明，使用本工作中应用的工艺和技术可以制备出适用于蓝藻毒素的基质参考物质。
