用于在十二烷基硫酸钠聚丙烯酰胺凝胶电泳中检测脂多糖的高灵敏度荧光染色剂。

Highly sensitive fluorescent stain for detecting lipopolysaccharides in sodium dodecyl sulfate polyacrylamide gel electrophoresis.

作者信息

Wang Xu, Zhou Ayi, Cai Wanhui, Yu Dongdong, Zhu Zhongxin, Jiang Chengxi, Jin Litai

机构信息

School of Pharmaceutical Sciences, Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.

Wenzhou Undersun Biotechnology Co., Ltd, Wenzhou, Zhejiang, P. R. China.

出版信息

Electrophoresis. 2015 Aug;36(15):1795-800. doi: 10.1002/elps.201500063. Epub 2015 Jul 1.

DOI:10.1002/elps.201500063

PMID:25930092

Abstract

A sensitive and simple technique was developed for the visualization of gel-separated lipopolysaccharides by using a hydrazide derivative, UGF202. As low as 0.5-1 ng total LPS could be detected by UGF202 stain, which is 2- and 16-fold more sensitive than that of the commonly used Pro-Q Emerald 300 and Keenan et al. developed silver stain, respectively. The results indicated that UGF202 stain could be a good choice for LPS determination in polyacrylamide gels.

摘要

通过使用酰肼衍生物UGF202，开发了一种灵敏且简单的技术，用于可视化凝胶分离的脂多糖。UGF202染色可检测低至0.5 - 1 ng的总脂多糖，其灵敏度分别比常用的Pro-Q Emerald 300和Keenan等人开发的银染色高2倍和16倍。结果表明，UGF202染色可能是聚丙烯酰胺凝胶中脂多糖测定的一个良好选择。