Hardy E, Pupo E, Castellanos-Serra L, Reyes J, Fernández-Patrón C
Center for Genetic Engineering and Biotechnology, Havana City, Cuba.
Anal Biochem. 1997 Jan 1;244(1):28-32. doi: 10.1006/abio.1996.9719.
We present a new method for visualizing bacterial lipopolysaccharides (LPS)/lipooligosaccharides (LOS) electrophoresed in sodium dodecyl sulfate-polyacrylamide gels. After electrophoresis, gels are washed in boiling water to appreciably remove remaining electrophoresis reagents, then incubated in 10 mM zinc sulfate for 15 min, and subsequently immersed in 0.2 M imidazole for 3 min. As a result, zinc salts precipitate all over the gel surface except in the zones occupied by LPS/LOS, which appear as transparent, colorless bands. Gels can be stored in distilled water for weeks without loss of the negative image. The sensitivity of this stain is similar to that of silver. We believe that zinc-imidazole may be a suitable nontoxic alternative to silver in the rapid analysis of LPS/LOS by polyacrylamide gel electrophoresis.
我们提出了一种新方法，用于可视化在十二烷基硫酸钠-聚丙烯酰胺凝胶中进行电泳的细菌脂多糖（LPS）/脂寡糖（LOS）。电泳后，将凝胶在沸水中洗涤以显著去除残留的电泳试剂，然后在10 mM硫酸锌中孵育15分钟，随后浸入0.2 M咪唑中3分钟。结果，除了被LPS/LOS占据的区域外，锌盐沉淀在整个凝胶表面，这些区域呈现为透明的无色条带。凝胶可以保存在蒸馏水中数周而不会损失负像。这种染色的灵敏度与银染色相似。我们认为，在通过聚丙烯酰胺凝胶电泳对LPS/LOS进行快速分析时，锌-咪唑可能是一种合适的无毒替代银的方法。
