Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou 325035, China.
Anal Biochem. 2012 Jul 1;426(1):1-3. doi: 10.1016/j.ab.2012.03.022. Epub 2012 Apr 4.
A sensitive and simple technique for the negative detection of lipopolysaccharides (LPSs) following polyacrylamide gel electrophoresis (PAGE) using eosin B (EB) was developed. After electrophoresis, gels were fixed, stained, and developed within 30 min to achieve transparent and colorless LPS bands under opaque gel matrix background. As low as 20 to 40 ng of total LPSs could be detected, which is 4-fold more sensitive than those of the widely used silver stain developed by Fomsgaard and coworkers and imidazole-zinc (IZ) negative stain. For its sensitivity and brevity, this stain may be a practical method for LPS determination in the routine laboratory.
开发了一种在聚丙烯酰胺凝胶电泳 (PAGE) 后使用曙红 B (EB) 对脂多糖 (LPS) 进行阴性检测的灵敏且简单的技术。电泳后，凝胶在 30 分钟内固定、染色和显色，在不透明的凝胶基质背景下实现透明无色的 LPS 带。低至 20 至 40ng 的总 LPS 即可被检测到，比 Fomsgaard 及其同事开发的广泛使用的银染法和咪唑锌 (IZ) 负染法灵敏 4 倍。由于其灵敏度和简洁性，这种染色法可能是常规实验室中 LPS 测定的实用方法。
