Xie Hongdong, Hou Wei, Yang Yide, Yu Ying, Wang Fengling, Mao Juanjuan
Department of Infectious Disease, Taizhou Municipal Hospital Taizhou 318000, China.
Int J Clin Exp Med. 2015 Jan 9;8(2):2226-32. eCollection 2015.
Traditional herbal medicine is usually administrated according to experiences and practices. We aimed to investigate the anti-fibrotic effects of Shenqi Neijin powder (SQNJP) in hepatic fibrosis rats induced by carbon tetrachloride (CCl4). A total of 32 rats were divided into control group, model group, and SQNJP group. The hydroxyproline content was assayed. Histological features of liver tissues were determined with different staining methods. Western blotting analysis and immuno-fluorescence staining were performed to determine the expression of alpha-smooth muscle actin (α-SMA) and the activation of hepatic stellate cells (HSCs). Serial sections were stained with α-SMA immuno-fluorescence staining and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method (TUNEL) in turn to detect the apoptosis of HSCs. Fatty degeneration, deposition of collagen, and interval of fibers were noticed in rats induced by CCl4. After administration of SQNJP, remarkable decrease of fatty degeneration, deposition of collagen, and hydroxyproline content were noticed. Compared with the model group, significant decrease of α-SMA protein was noticed after administration of SQNJP, and remarkable apoptosis of HSCs was noticed after treating with SQNJP. SQNJP showed anti-fibrotic effects through inhibiting HSCs activation and inducing apoptosis of HSCs.
传统草药通常根据经验和实践来使用。我们旨在研究参芪内金散（SQNJP）对四氯化碳（CCl4）诱导的肝纤维化大鼠的抗纤维化作用。总共32只大鼠被分为对照组、模型组和参芪内金散组。检测羟脯氨酸含量。用不同的染色方法确定肝组织的组织学特征。进行蛋白质印迹分析和免疫荧光染色以确定α-平滑肌肌动蛋白（α-SMA）的表达和肝星状细胞（HSCs）的活化。连续切片依次用α-SMA免疫荧光染色和末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法（TUNEL）染色以检测肝星状细胞的凋亡。在CCl4诱导的大鼠中观察到脂肪变性、胶原沉积和纤维间隔。给予参芪内金散后，观察到脂肪变性、胶原沉积和羟脯氨酸含量显著降低。与模型组相比，给予参芪内金散后α-SMA蛋白显著降低，用参芪内金散治疗后观察到肝星状细胞明显凋亡。参芪内金散通过抑制肝星状细胞活化和诱导肝星状细胞凋亡发挥抗纤维化作用。
