Umemoto S, Kodaira S, Abe O, Takami H
Nihon Shokakibyo Gakkai Zasshi. 1989 Oct;86(10):2379-86.
The administration of theophylline (120 mg/kg.day, 14 wk) to the drinking water of female BALB/c mice after treatment with 1,2-dimethylhydrazine (DMH) (30 mg/kg.sc, 1/wk, 14 wk) resulted in both an increase in number of colon carcinoma and increases in ornithine decarboxylase (ODC) activities and polyamine (PA) levels in colon tissue. This increase in number of colon carcinoma was about 3-fold to mice receiving same amount of DMH and drinking water without theophylline. ODC activities (pmoles 14CO2/hr/mg prot, mean +/- SD) in colon tissue were 122 +/- 10.6 (n = 6) in DMH with theophylline group, 28.3 +/- 2.13 (n = 8) in DMH alone group, 21.3 +/- 1.67 (n = 6) in control group respectively. Putrescine and spermidine levels (nmoles/g, mean +/- SD) were 31.0 +/- 10.5, 527 +/- 86.6 (n = 11) in DMH with theophylline group, 24.0 +/- 11.4, 464 +/- 129 (n = 11) in control group respectively. Thus with marked increase of ODC activities and slight increase of PA levels, theophylline has shown to significantly enhance the promoting phase of carcinogenic process with DMH.
在用1,2 - 二甲基肼（DMH）（30mg/kg，皮下注射，每周1次，共14周）处理雌性BALB/c小鼠后，给其饮用水中添加茶碱（120mg/kg·天，共14周），结果显示结肠癌数量增加，同时结肠组织中的鸟氨酸脱羧酶（ODC）活性和多胺（PA）水平也升高。与接受相同剂量DMH但饮用不含茶碱的水的小鼠相比，结肠癌数量增加了约3倍。结肠组织中的ODC活性（每小时每毫克蛋白产生的14CO2的皮摩尔数，平均值±标准差）在DMH加茶碱组为122±10.6（n = 6），单独DMH组为28.3±2.13（n = 8），对照组为21.3±1.67（n = 6）。腐胺和亚精胺水平（纳摩尔/克，平均值±标准差）在DMH加茶碱组分别为31.0±10.5、527±86.6（n = 11），对照组分别为24.0±11.4、464±129（n = 11）。因此，随着ODC活性显著增加和PA水平略有增加，茶碱已显示出能显著增强DMH致癌过程的促进阶段。
