Amano T, Umakoshi Y, Kuwajima N, Mino Y, Takahashi K, Suzuki S, Ota Z, Huang S
Nihon Jinzo Gakkai Shi. 1989 Aug;31(8):807-13.
In order to examine the ability of complement to solubilize the immune complexes (IC) in vivo, we made acute or chronic serum sickness nephritis in 10 rabbits or 12 rats with injection of BSA as antigen (Ag). Animals were divided into two groups. The 500 mu or 50 mu/week of cobra venom factor (CVF) for rabbits or rats was administered intraperitoneally in one group of animals to reduce the serum complement levels and complement-mediated solubilization of immune complex. On the other hand, the 2 ml or 0.2 ml/week of turpentine oil (TO) was injected intramuscularly in another group of animals to elevate the serum complement levels. The renal biopsy was performed with weekly intervals in each animals, and the specimens were stained for antigen (BSA), antibody (IgG) and C3 by immunofluorescence techniques. The result in this experiments showed the significant difference between TO-, and CVF-treated animals in the degree of disappearance of IC in the kidney lesions. The undetectable amount of IC (especially BSA) was remained in the renal tissue of TO-treated animals. This fact indicated that complement might act to solubilize the IC deposited in the tissue in vivo also.
为了检测补体在体内溶解免疫复合物（IC）的能力，我们给10只兔子或12只大鼠注射牛血清白蛋白（BSA）作为抗原（Ag），制造急性或慢性血清病肾炎。动物被分为两组。一组动物腹腔注射眼镜蛇毒因子（CVF），兔子每周500μ或大鼠每周50μ，以降低血清补体水平及补体介导的免疫复合物溶解。另一方面，另一组动物肌肉注射松节油（TO），兔子每周2ml或大鼠每周0.2ml，以提高血清补体水平。对每只动物每隔一周进行一次肾活检，标本采用免疫荧光技术进行抗原（BSA）、抗体（IgG）和C3染色。本实验结果显示，在肾脏病变中，经TO处理和经CVF处理的动物在IC消失程度上存在显著差异。在经TO处理的动物肾组织中，检测不到IC（尤其是BSA）。这一事实表明，补体在体内可能也起到溶解沉积在组织中的IC的作用。
