Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
ACS Nano. 2015 May 26;9(5):4950-6. doi: 10.1021/nn5069622. Epub 2015 May 7.
RNA nanotechnology has been established by employing the molecular architecture of RNA structural motifs. Here, we report two designed RNA-protein complexes (RNPs) composed of ribosomal protein L1 (RPL1) and its RNA-binding motif that are square-shaped nano-objects. The formation and the shape of the objects were confirmed by gel electrophoresis analysis and atomic force microscopy, respectively. Any protein can be attached to the RNA via a fusion protein with RPL1, indicating that it can be used as a scaffold for loading a variety of functional proteins or for building higher-order structures. In summary, the RNP object will serve as a useful tool in the fields of bionanotechnology and synthetic biology. Moreover, the RNP interaction enhances the RNA stability against nucleases, rendering these complexes stable in cells.
RNA 纳米技术是通过利用 RNA 结构基序的分子结构来实现的。在这里，我们报告了两种由核糖体蛋白 L1（RPL1）及其 RNA 结合基序组成的方形纳米级 RNA-蛋白质复合物（RNP）。通过凝胶电泳分析和原子力显微镜分别确认了物体的形成和形状。任何蛋白质都可以通过与 RPL1 融合的蛋白质与 RNA 结合，这表明它可以用作加载各种功能蛋白的支架，或用于构建更高阶结构。总之，RNP 物体将成为生物纳米技术和合成生物学领域的有用工具。此外，RNP 相互作用增强了 RNA 对核酸酶的稳定性，使这些复合物在细胞中稳定。
