用于RNA毛细管电泳的聚环氧乙烷（PEO）和聚乙二醇（PEG）聚合物筛分基质
Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis.
作者信息
Yamaguchi Yoshinori, Li Zhenqing, Zhu Xifang, Liu Chenchen, Zhang Dawei, Dou Xiaoming
机构信息
Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai 200237, China. Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai 200093, China; Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka, Suita-city, Osaka, 565-0871, Japan.
Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai 200237, China.
出版信息
PLoS One. 2015 May 1;10(5):e0123406. doi: 10.1371/journal.pone.0123406. eCollection 2015.
The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the separation performance for the large ones (>4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (Nmin) from the resolution length analysis.
选择用于通过毛细管电泳分离RNA片段的筛分聚合物至关重要。我们研究了在不同分子量和不同浓度的聚乙二醇（PEG）和聚环氧乙烷（PEO）溶液中100至10,000 nt范围内RNA片段的分离情况。我们发现，小分子RNA片段（<1000 nt）的分离性能随着聚合物浓度的增加而提高，而当PEG/PEO溶液中浓度分别高于1.0%/0.6%时，大分子RNA片段（>4000 nt）的分离性能会变差。通过迁移率与RNA片段大小的双对数图，我们揭示了RNA在PEG（300 - 500k）和PEO（4,000k）中的三种迁移模式。此外，我们通过分辨率长度分析计算出了最小可分辨核苷酸长度（Nmin）。
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