Yenepoya Research Centre, Yenepoya University, Mangalore, India.
Yenepoya Research Centre, Yenepoya University, Mangalore, India.
Carbohydr Polym. 2015 Aug 1;126:215-21. doi: 10.1016/j.carbpol.2015.03.017. Epub 2015 Mar 17.
Exopolysaccharides (EPS) produced by bacteria have attracted scientific and industrial attention due to their multifunctional properties and relatively easier production. In this study, an EPS viz., R-PS18 produced by Rhizobium sp. PRIM-18 was characterized and its functional properties were assessed. Cell proliferative and in vitro wound healing activities of the EPS were established using human dermal fibroblast (HDF) cells. The isolate produced 2.1 g L(-1) purified EPS (molecular weight 9.33×10(6) Da) comprising of glucose, galactose, and mannose (6.1:1.8:1). Viscosity of 0.25% solution was 23.4 mPa s (shear rate 75 s(-1)) and it showed pseudoplastic and thixotropic behavior. High emulsification, iron chelation, and superoxide scavenging abilities were also observed. Significant increase in HDF cell proliferation and wound healing in vitro was achieved by R-PS18 treatment. Sulfation of R-PS18 significantly enhanced the cell proliferative and wound healing activities. In conclusion, these findings indicate potential applications of R-PS18.
细菌产生的胞外多糖 (EPS) 因其多功能特性和相对更容易生产而引起了科学界和工业界的关注。在这项研究中，我们对 Rhizobium sp. PRIM-18 产生的 EPS（即 R-PS18）进行了表征，并评估了其功能特性。我们使用人真皮成纤维细胞 (HDF) 细胞来确定 EPS 的细胞增殖和体外伤口愈合活性。该分离株产生了 2.1 g L(-1) 的纯化 EPS（分子量为 9.33×10(6) Da），由葡萄糖、半乳糖和甘露糖（6.1:1.8:1）组成。0.25%溶液的黏度为 23.4 mPa s（剪切速率为 75 s(-1))，表现出假塑性和触变性。同时还观察到高乳化、铁螯合和超氧阴离子清除能力。R-PS18 处理可显著增加 HDF 细胞的增殖和体外伤口愈合。R-PS18 的硫酸化显著增强了细胞增殖和伤口愈合活性。总之，这些发现表明 R-PS18 具有潜在的应用前景。
