Departamento de Microbioloxía e Parasitoloxía, Facultade de Bioloxía, Centro de Investigación Biolóxica (CIBUS), Universidade de Santiago de Compostela, Campus Vida, Santiago de Compostela, 15782, Spain.
CPIRN-UDI/IPG, Centro de Potencial e Inovação em Recursos Naturais, Unidade de Investigação para o Desenvolvimento do Interior do Instituto Politécnico da Guarda, Portugal.
Carbohydr Polym. 2021 Feb 15;254:117303. doi: 10.1016/j.carbpol.2020.117303. Epub 2020 Oct 24.
Exopolysaccharides (EPS) produced by cyanobacteria are complex biomolecules of anionic nature with potential biomedical applications. In this study, the EPS produced by the Nostoc sp. strains PCC7936 and PCC7413 were characterized and evaluated as a biomaterial for new wound dressings. The addition of acetate ions to the culture medium slightly stimulated EPS production, achieving 1463.1 ± 16.0 mgL (PCC7413) and 1372.1 ± 29.0 mgL (PCC7936). Both EPS presented nine monosaccharide residues and a MW > 1000 kDa. The acetate addition changed the monosaccharide molar percentages. FTIR and DLS results confirmed the anionic nature and the presence of sulfate groups in both EPS, which are determinant features for biomedical applications. Both EPS at 1%(w/v) formed gels in the presence of 0.4%(w/v) FeCl. Results obtained for MTT assay and wound healing in vitro scratch assay revealed hydrogels biocompatibility and ability to promote fibroblast migration and proliferation that was greater in PCC7936. The Nostoc EPS hydrogels presented promising properties to be applied in the treatment of skin injuries.
蓝藻产生的胞外多糖（EPS）是具有阴离子性质的复杂生物分子，具有潜在的生物医学应用。在这项研究中，对 Nostoc sp. 菌株 PCC7936 和 PCC7413 产生的 EPS 进行了表征，并将其评估为新型伤口敷料的生物材料。向培养基中添加乙酸盐略微刺激了 EPS 的产生，分别达到 1463.1 ± 16.0 mg/L（PCC7413）和 1372.1 ± 29.0 mg/L（PCC7936）。两种 EPS 都含有九个单糖残基和 MW>1000 kDa。乙酸盐的添加改变了单糖摩尔百分比。FTIR 和 DLS 结果证实了两种 EPS 的阴离子性质和硫酸盐基团的存在，这是生物医学应用的决定因素。在存在 0.4%（w/v）FeCl 的情况下，1%（w/v）的两种 EPS 都形成了凝胶。MTT 测定和体外划痕愈合试验的结果表明水凝胶具有生物相容性，能够促进成纤维细胞的迁移和增殖，PCC7936 的效果更好。Nostoc EPS 水凝胶具有应用于皮肤损伤治疗的有前景的特性。
