Hagen Jana, Scheerlinck Jean-Pierre Y, Gasser Robin B
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria 3010, Australia.
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria 3010, Australia.
Trends Parasitol. 2015 Jul;31(7):324-32. doi: 10.1016/j.pt.2015.03.009. Epub 2015 Apr 28.
Underpinned by major advances in our understanding of the genomes of schistosomes, progress in the development of functional genomic tools is providing unique prospects to gain insights into the intricacies of the biology of these blood flukes, their host relationships, and the diseases that they cause. This article reviews some key applications of double-stranded RNA interference (RNAi) in Schistosoma mansoni, appraises delivery systems for transgenesis and stable gene silencing, considers ways of increasing efficiency and specificity of gene silencing, and discusses the prospects of using a lentivirus delivery system for future functional genomic-phenomic explorations of schistosomes and other parasites. The ability to achieve effective and stable gene perturbation in parasites has major biological implications and could facilitate the development of new interventions.
在我们对血吸虫基因组理解取得重大进展的支撑下，功能基因组工具的开发进展为深入了解这些血吸虫的生物学复杂性、它们与宿主的关系以及它们所引发的疾病提供了独特的前景。本文综述了双链RNA干扰（RNAi）在曼氏血吸虫中的一些关键应用，评估了转基因和稳定基因沉默的递送系统，探讨了提高基因沉默效率和特异性的方法，并讨论了使用慢病毒递送系统对血吸虫和其他寄生虫进行未来功能基因组-表型组学探索的前景。在寄生虫中实现有效且稳定的基因干扰的能力具有重大生物学意义，并可能促进新干预措施的开发。
