Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Biomaterials. 2015 Jul;56:86-91. doi: 10.1016/j.biomaterials.2015.03.051. Epub 2015 Apr 16.
A large number of lineage-committed progenitor cells are required for advanced regenerative medicine based on cell engineering. Due to their ability to differentiate into multiple cells lines, multipotent stem cells have emerged as a vital source for generating transplantable cells for use in regenerative medicine. Increment in differentiation efficiency of the mesenchymal stem cell was obtained by using hydrogel to adjust the proliferation cycle of encapsulated cells to signal sensitive phase. Three dimensional (3-D) polymer networks composed of poly(2-methacyloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-p-vinylphenylboronic acid (VPBA)) (PMBV) and poly(vinyl alcohol) (PVA) were prepared as a hydrogel. The proliferation of cells encapsulated in the PMBV/PVA hydrogel was highly sensitive to the storage modulus (G') of the hydrogel. That is, when the G' value of the hydrogel was higher than 1.0 kPa, the cell proliferation was ceased and the proliferation cycle of cells was converged to G1 phase, whereas when the G' value was below 1.0 kPa, cell proliferation proceeded. By changing the G' value of hydrogels under encapsulation the cells, proliferation cycle of encapsulated mesenchymal stem cells was regulated to G1 phase and thus signal sensitivity were increased. 3-D polymer networks as hydrogels with tunable physical properties can be effectively used to control proliferation and lineage-restricted differentiation of stem cells.
大量的谱系定向祖细胞是基于细胞工程的先进再生医学所必需的。由于多能干细胞能够分化为多种细胞系，因此它们已成为生成可用于再生医学的移植细胞的重要来源。通过使用水凝胶来调整包封细胞的增殖周期到信号敏感阶段，从而提高间充质干细胞的分化效率。由聚（2-甲基丙烯酰氧基乙基磷酸胆碱（MPC）-共-正丁基甲基丙烯酸酯（BMA）-共-p-乙烯基苯硼酸（VPBA））（PMBV）和聚乙烯醇（PVA）组成的三维（3-D）聚合物网络被制备为水凝胶。包封在 PMBV/PVA 水凝胶中的细胞的增殖对水凝胶的储能模量（G'）高度敏感。也就是说，当水凝胶的 G'值高于 1.0kPa 时，细胞增殖停止，细胞增殖周期收敛到 G1 期，而当 G'值低于 1.0kPa 时，细胞增殖继续。通过改变包封细胞下水凝胶的 G'值，调节包封间充质干细胞的增殖周期到 G1 期，从而提高信号敏感性。具有可调物理性质的 3-D 聚合物网络作为水凝胶可以有效地用于控制干细胞的增殖和谱系限制分化。
