Alio Sanda Abdelkader, Granger Farbos Alexandra, Hamidou Amina Amadou, Vonaesch Pascale, Jusot Jean-François, Koeck Jean-Louis, Collard Jean-Marc
Centre de Recherche Médicale et Sanitaire (CERMES), Niamey, Niger.
Laboratoire de Biologie Moléculaire, Hôpital d'Instruction des Armées Robert-Picqué, Bordeaux, France.
Trans R Soc Trop Med Hyg. 2015 Jul;109(7):477-80. doi: 10.1093/trstmh/trv033. Epub 2015 May 1.
Serotype 1 was the most prevalent pneumococcal meningitis serotype encountered in Niger over the period 2003-2011 (pre-vaccination era), accounting for 45.3% of infections.
Multiple locus VNTR analysis (MLVA) was used to create a genotypic snapshot of a representative subset of the pneumococcal population of serotype 1.
MLVA using 16 markers revealed a homogeneous genetic background of pneumococci serotype 1 from Niger, which clustered with few serotype 1 pneumococci from some African countries, while other African countries displayed different clonal complexes. DNA from Niger and from other African countries were different from pneumococci serotype 1 from European countries.
MLVA-typing revealed a low genetic diversity among pneumococci serotype 1 from meningitis cases in Niger in the pre-vaccination era.
在2003年至2011年期间（疫苗接种前时代），血清型1是尼日尔最常见的肺炎球菌性脑膜炎血清型，占感染病例的45.3%。
采用多位点可变数目串联重复序列分析（MLVA）对血清型1肺炎球菌群体的一个代表性子集进行基因分型。
使用16个标记的MLVA显示，来自尼日尔的血清型1肺炎球菌具有同质的遗传背景，与一些非洲国家的少数血清型1肺炎球菌聚类在一起，而其他非洲国家则显示出不同的克隆复合体。来自尼日尔和其他非洲国家的DNA与来自欧洲国家的血清型1肺炎球菌不同。
MLVA分型显示，在疫苗接种前时代，尼日尔脑膜炎病例中血清型1肺炎球菌的遗传多样性较低。
