Syed-Hussain S S, Howe L, Pomroy W E, West D M, Hardcastle M, Williamson N B
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; Institute of Veterinary Animal and Biomedical Sciences, Massey University, Palmerston North 4412, New Zealand.
Institute of Veterinary Animal and Biomedical Sciences, Massey University, Palmerston North 4412, New Zealand.
Vet Parasitol. 2015 Jun 15;210(3-4):141-4. doi: 10.1016/j.vetpar.2015.03.019. Epub 2015 Apr 1.
To determine if toltrazuril was effective in eliminating Neospora caninum infection from congenitally infected lambs. Twenty-eight ewes were allocated to 3 groups where animals in Groups A and B were inoculated with 1 × 10(7)N. caninum tachyzoites on Day 120 of gestation and Group C was maintained as a negative control group. Lambs born from ewes in Group A were treated with toltrazuril (20mg/kg) on Days 0, 7, 14 and 21 after birth. Lambs in Groups B and C were untreated. All lambs in Groups A and B were seropositive at 12 weeks of age. At 12 weeks of age, no differences between lambs in Group A and Group B were observed in serological results (ELISA and western blot), presence of N. caninum-related brain histopathological lesions or the number of organisms detected by qPCR. Group C remained negative for serology, detection of N. caninum DNA as well as histopathology throughout the study. Results indicate that N. caninum congenitally-infected lambs had a continuing infection with N. caninum despite being treated with toltrazuril.
为了确定托曲珠利是否能有效清除先天性感染新孢子虫的羔羊体内的新孢子虫感染。28只母羊被分为3组，A组和B组的动物在妊娠第120天接种1×10(7)个新孢子虫速殖子，C组作为阴性对照组。A组母羊所生的羔羊在出生后第0、7、14和21天用托曲珠利（20mg/kg）进行治疗。B组和C组的羔羊未接受治疗。A组和B组的所有羔羊在12周龄时血清学检测均为阳性。在12周龄时，A组和B组羔羊在血清学结果（酶联免疫吸附测定和免疫印迹法）、新孢子虫相关脑组织病理学病变的存在情况或通过定量聚合酶链反应检测到的病原体数量方面均未观察到差异。在整个研究过程中，C组血清学、新孢子虫DNA检测以及组织病理学检查均为阴性。结果表明，尽管用托曲珠利进行了治疗，但先天性感染新孢子虫的羔羊仍持续感染新孢子虫。
