College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, PR China; Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China; Lancaster Environment Centre, Lancaster University, Lancaster LA1 2YQ, UK.
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China.
Bioresour Technol. 2015 Aug;190:82-8. doi: 10.1016/j.biortech.2015.04.068. Epub 2015 Apr 23.
This study developed a magnetic nanoparticles (MNPs) harvesting and reactivation technique for rapid cyanobacteria Microcystis aeruginosa separation. The harvesting of raw MNPs achieved high efficiency of 99.6% with the MNPs dosage of 0.58g MNPs/g dry-biomass, but gradually decreased to 59.1% when directly reused 5 times. With extra ultrasonic chloroform:methanol solvent treatment, the MNPs can be effectively reactivated for M. aeruginosa harvesting with 60% efficiency after 5 times reactivation and the separation efficiency kept above 93% with 0.20g MNPs/g dry-biomass dosage. The cyanobacteria-MNPs complex can be effectively disrupted by ultrasonic chloroform:methanol solvent treatment and the zeta potential was recovered for MNPs electrostatic attraction. The MNPs adsorption followed the Langmuir isotherm, and the maximum adsorption capacity and Langmuir constant was 3.74g dry-biomass/g and 311.64L/g respectively. This MNPs reactivation technique can achieve low energy separation and reduce MNPs consumption by 67%, providing potential engineering implementation for cyanobacterial biomass harvesting.
本研究开发了一种磁性纳米颗粒（MNPs）收获和再激活技术，用于快速分离蓝藻铜绿微囊藻。在 MNPs 用量为 0.58g MNPs/g 干生物质的情况下，原始 MNPs 的收获效率高达 99.6%，但直接重复使用 5 次后逐渐降至 59.1%。经过额外的超声氯仿：甲醇溶剂处理，MNPs 可以有效地再激活，在 5 次再激活后，对铜绿微囊藻的收获效率达到 60%，且在 0.20g MNPs/g 干生物质用量下，分离效率保持在 93%以上。超声氯仿：甲醇溶剂处理可以有效地破坏蓝藻-MNPs 复合物，并恢复 MNPs 的静电吸引力的zeta 电位。MNPs 的吸附遵循 Langmuir 等温线，最大吸附容量和 Langmuir 常数分别为 3.74g 干生物质/g 和 311.64L/g。这种 MNPs 再激活技术可以实现低能耗分离，并将 MNPs 的消耗减少 67%，为蓝藻生物质的收获提供了潜在的工程实施途径。
