对从家蚕幼虫中纯化的重组犬新孢子虫抗原进行评估，以保护小鼠免受犬新孢子虫感染。
Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice.
作者信息
Yoshimoto Mai, Otsuki Takahiro, Itagaki Kohei, Kato Tatsuya, Kohsaka Tetsuya, Matsumoto Yumino, Ike Kazunori, Park Enoch Y
机构信息
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
出版信息
J Biosci Bioeng. 2015 Dec;120(6):715-9. doi: 10.1016/j.jbiosc.2015.04.002. Epub 2015 Apr 29.
Three antigens (NcSAG1, NcSRS2 and NcMIC3) from Neospora caninum were expressed using the BmNPV bacmid system in silkworm larvae and purified from the hemolymph. From 20 silkworm larvae, 1.5, 1.2 and 1.4 mg of purified recombinant NcSAG1, NcSRS2 and NcMIC3 were obtained, respectively. When each purified recombinant antigen was immunized with Freund's incomplete adjuvant (FIA) to mice, recombinant NcSAG1 induced a Th2 immune response in immunized mice and produced a SAG1-specific antibody. In the experiment where NcSAG1-immunized mice were challenged with N. caninum, the cerebral N. caninum burden was significantly reduced compared with that of either the FIA- or PBS-immunized mice. Recombinant NcSRS2 or NcMIC3 induced both Th1 and Th2 immune responses, but NcMIC3-immunization did not induce significant production of NcMIC3-specific antibodies. These results suggest that the silkworm can produce recombinant antigens of N. caninum, which can be used as a recombinant vaccine against N. caninum.
利用家蚕杆状病毒杆粒系统在家蚕幼虫中表达了犬新孢子虫的三种抗原（NcSAG1、NcSRS2和NcMIC3），并从血淋巴中进行了纯化。从20只家蚕幼虫中分别获得了1.5毫克、1.2毫克和1.4毫克纯化的重组NcSAG1、NcSRS2和NcMIC3。当用弗氏不完全佐剂（FIA）将每种纯化的重组抗原免疫小鼠时，重组NcSAG1在免疫小鼠中诱导了Th2免疫反应，并产生了SAG1特异性抗体。在用犬新孢子虫攻击NcSAG1免疫小鼠的实验中，与FIA或PBS免疫小鼠相比，脑内犬新孢子虫负荷显著降低。重组NcSRS2或NcMIC3诱导了Th1和Th2免疫反应，但NcMIC3免疫未诱导产生显著的NcMIC3特异性抗体。这些结果表明，家蚕能够生产犬新孢子虫的重组抗原，可作为抗犬新孢子虫的重组疫苗。
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