Avagyan Rozanna, Nyström Robin, Boman Christoffer, Westerholm Roger
Department of Environmental Science and Analytical Chemistry, Arrhenius Laboratory, Stockholm University, 10691, Stockholm, Sweden.
Anal Bioanal Chem. 2015 Jun;407(16):4523-34. doi: 10.1007/s00216-015-8638-x. Epub 2015 May 3.
A simple and fast method for analysis of hydroxylated polycyclic aromatic hydrocarbons using pressurized liquid extraction and high performance liquid chromatography utilizing photoionization tandem mass spectrometry was developed. Simultaneous separation and determination of nine hydroxylated polycyclic aromatic hydrocarbons and two hydroxy biphenyls could be performed in negative mode with a run time of 12 min, including equilibration in 5 min. The calibration curves were in two concentration ranges; 1-50 ng/mL and 0.01-50 μg/mL, with coefficients of correlation R (2) > 0.997. The limits of detection and method quantification limits were in the range of 9-56 pg and 5-38 ng/g, respectively. A two-level full factorial experimental design was used for screening of conditions with the highest impact on the extraction. The extraction procedure was automated and suitable for a large number of samples. The extraction recoveries ranged from 70 to 102 % and the matrix effects were between 92 and 104 %. The overall method was demonstrated on wood smoke particles and soil samples with good analytical performance, and five OH-PAHs were determined in the concentration range of 0.19-210 μg/g. As far as we know, hydroxylated polycyclic aromatic hydrocarbons were determined in wood smoke and soil samples using photoionization mass spectrometry for the first time in this present study. Accordingly, this study shows that high performance liquid chromatography photoionization tandem mass spectrometry can be a good option for the determination of hydroxylated polycyclic aromatic hydrocarbons in complex environmental samples. Graphical Abstract The method developed in this study was used to determine hydroxylated polycyclic aromatic hydrocarbons in wood smoke and soil.
开发了一种简单快速的方法，用于分析羟基化多环芳烃，该方法采用加压液体萃取和利用光电离串联质谱的高效液相色谱法。在负离子模式下，12分钟内可同时分离和测定9种羟基化多环芳烃和2种羟基联苯，包括5分钟的平衡时间。校准曲线在两个浓度范围内；1 - 50 ng/mL和0.01 - 50 μg/mL，相关系数R(2)＞0.997。检测限和方法定量限分别在9 - 56 pg和5 - 38 ng/g范围内。采用两级全因子实验设计筛选对萃取影响最大的条件。萃取过程自动化，适用于大量样品。萃取回收率在70%至102%之间，基质效应在92%至104%之间。该方法在木烟颗粒和土壤样品上得到验证，具有良好的分析性能，测定了5种羟基多环芳烃，浓度范围为0.19 - 210 μg/g。据我们所知，本研究首次使用光电离质谱法测定木烟和土壤样品中的羟基化多环芳烃。因此，本研究表明，高效液相色谱光电离串联质谱法是测定复杂环境样品中羟基化多环芳烃的一个很好的选择。图形摘要 本研究开发的方法用于测定木烟和土壤中的羟基化多环芳烃。
