Amini Ryan, Zhang Zijie, Li Jiuxing, Gu Jimmy, Brennan John D, Li Yingfu
Department of Biochemistry and Biomedical Sciences McMaster University 1280 Main Street West Hamilton Ontario L8S 4K1 Canada.
Biointerfaces Institute McMaster University 1280 Main Street West Hamilton Ontario L8S 4K1 Canada.
Anal Sens. 2022 Sep;2(5):e202200012. doi: 10.1002/anse.202200012. Epub 2022 May 12.
The SARS-CoV-2 virus and COVID-19 pandemic continue to demand effective diagnostic and therapeutic solutions. Finding these solutions requires highly functional molecular recognition elements. Nucleic acid aptamers represent a possible solution. Characterized by their high affinity and specificity, aptamers can be rapidly identified from random-sequence nucleic acid libraries. Over the past two years, many labs around the world have rushed to create diverse aptamers that target two important structural proteins of SARS-CoV-2: the spike (S) protein and nucleocapsid (N) protein. These have led to the identification of many aptamers that show real promise for the development of diagnostic tests and therapeutic agents for SARS-CoV-2. Herein we review all these developments, with a special focus on the development of diverse aptasensors for detecting SARS-CoV-2. These include electrochemical and optical sensors, lateral flow devices, and aptamer-linked immobilized sorbent assays.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒和新型冠状病毒肺炎（COVID-19）大流行持续需要有效的诊断和治疗解决方案。找到这些解决方案需要高功能的分子识别元件。核酸适配体是一种可能的解决方案。适配体具有高亲和力和特异性的特点，可从随机序列核酸文库中快速鉴定出来。在过去两年里，世界各地的许多实验室纷纷竞相制备针对SARS-CoV-2两种重要结构蛋白的各种适配体：刺突（S）蛋白和核衣壳（N）蛋白。这些工作已鉴定出许多适配体，它们在开发SARS-CoV-2诊断测试和治疗剂方面显示出真正的前景。在此，我们综述所有这些进展，特别关注用于检测SARS-CoV-2的各种适配体传感器的开发。这些包括电化学和光学传感器、侧向流动装置以及适配体连接的固定化吸附剂测定法。
