School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Int J Biol Macromol. 2015 Aug;79:72-5. doi: 10.1016/j.ijbiomac.2015.04.054. Epub 2015 Apr 30.
Lytic polysaccharide monooxygenases (LPMOs) are recently discovered oxidative enzymes that are capable of oxidative cleavage of recalcitrant polysaccharides such as chitin or cellulose. Despite the importance of LPMOs in biomass conversion and the large number of lpmo genes in microorganisms, only a few LPMOs have been well studied, and further characterization of these proteins is thus of interest. In this study, a chitin-active AA10 family LPMO from Bacillus thuringiensis subsp. kurstaki, BtLPMO10A, is described. This enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin, however, interestingly, when colloidal chitin is used as the substrate, a ladder of oxidized oligosaccharides is observed. These results provide new insights into the action mode of LPMOs that may be affected by the substrates.
溶细胞多糖单加氧酶（LPMOs）是最近发现的能够氧化分解几丁质或纤维素等难处理多糖的氧化酶。尽管 LPMOs 在生物质转化中具有重要意义，并且微生物中存在大量的 lpmo 基因，但只有少数 LPMOs 得到了很好的研究，因此进一步表征这些蛋白质很有意义。在本研究中，描述了来自苏云金芽孢杆菌亚种的一种具有几丁质活性的 AA10 家族 LPMO，BtLPMO10A。该酶从结晶几丁质产生偶数个氧化寡糖作为主要产物，然而，有趣的是，当使用胶体几丁质作为底物时，会观察到氧化寡糖的阶梯。这些结果为 LPMOs 的作用模式提供了新的见解，该模式可能会受到底物的影响。
