• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

球毛壳菌重组辅助活性9与纤维素酶在纤维素水解中协同作用的优化

Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis.

作者信息

Kim In Jung, Nam Ki Hyun, Yun Eun Ju, Kim Sooah, Youn Hak Jin, Lee Hee Jin, Choi In-Geol, Kim Kyoung Heon

机构信息

Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.

Pohang Accelerator Laboratory, Pohang University of Science and Technology, Pohang, 790-784, Republic of Korea.

出版信息

Appl Microbiol Biotechnol. 2015 Oct;99(20):8537-47. doi: 10.1007/s00253-015-6592-3. Epub 2015 May 5.

DOI:10.1007/s00253-015-6592-3
PMID:25936375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4768223/
Abstract

Auxiliary activity family 9 (AA9, formerly known as glycoside hydrolase family 61 or polysaccharide monooxygenase) is a group of fungal proteins that were recently found to have a significant synergism with cellulase in cellulose hydrolysis via the oxidative cleavage of glycosidic bonds of cellulose chains. In this study, we report the active expression of a recombinant fungal AA9 from Chaetomium globosum (CgAA9) in a bacterial host, Escherichia coli, and the optimization of its synergistic activity in cellulose hydrolysis by using cellulase. The recombinant CgAA9 (0.9 mg/g cellulose) exhibited 1.7-fold synergism in the hydrolysis of Avicel when incubated with 0.9 filter paper units of Celluclast 1.5 L/g cellulose. The first study of the active expression of AA9 using a bacterial host and its synergistic optimization could be useful for the industrial application of AA9 for the saccharification of lignocellulose.

摘要

辅助活性家族9（AA9，以前称为糖苷水解酶家族61或多糖单加氧酶）是一组真菌蛋白，最近发现它们通过氧化裂解纤维素链的糖苷键，在纤维素水解过程中与纤维素酶具有显著的协同作用。在本研究中，我们报道了来自球毛壳菌的重组真菌AA9（CgAA9）在细菌宿主大肠杆菌中的活性表达，以及通过使用纤维素酶对其在纤维素水解中的协同活性进行优化。当与0.9滤纸单位的纤维素酶Celluclast 1.5 L/g纤维素一起孵育时，重组CgAA9（0.9 mg/g纤维素）在微晶纤维素水解中表现出1.7倍的协同作用。首次利用细菌宿主对AA9进行活性表达及其协同优化的研究，可能有助于AA9在木质纤维素糖化工业应用中的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/d94e21d0b21b/253_2015_6592_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/e5679fbf1b43/253_2015_6592_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/bcdfe75a2bd9/253_2015_6592_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/acdbd8283fe2/253_2015_6592_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/d95d4d293b08/253_2015_6592_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/f5666ae81270/253_2015_6592_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/458753b2f20b/253_2015_6592_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/d94e21d0b21b/253_2015_6592_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/e5679fbf1b43/253_2015_6592_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/bcdfe75a2bd9/253_2015_6592_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/acdbd8283fe2/253_2015_6592_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/d95d4d293b08/253_2015_6592_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/f5666ae81270/253_2015_6592_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/458753b2f20b/253_2015_6592_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a17/4768223/d94e21d0b21b/253_2015_6592_Fig7_HTML.jpg

相似文献

1
Optimization of synergism of a recombinant auxiliary activity 9 from Chaetomium globosum with cellulase in cellulose hydrolysis.球毛壳菌重组辅助活性9与纤维素酶在纤维素水解中协同作用的优化
Appl Microbiol Biotechnol. 2015 Oct;99(20):8537-47. doi: 10.1007/s00253-015-6592-3. Epub 2015 May 5.
2
Identification and Characterization of Two Novel Extracellular β-Glucanases from Chaetomium globosum against Fusarium sporotrichioides.从卷枝毛霉中鉴定和表征两种新型的胞外β-葡聚糖酶对抗匐枝根霉。
Appl Biochem Biotechnol. 2024 Jun;196(6):3199-3215. doi: 10.1007/s12010-023-04698-1. Epub 2023 Aug 29.
3
Identification and characterization of a novel AA9-type lytic polysaccharide monooxygenase from a bagasse metagenome.从甘蔗渣宏基因组中鉴定和表征一种新型 AA9 型溶菌多糖单加氧酶。
Appl Microbiol Biotechnol. 2021 Jan;105(1):197-210. doi: 10.1007/s00253-020-11002-2. Epub 2020 Nov 24.
4
Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification.球毛壳菌纤维素酶系统对稻草糖化的生化特性及协同作用
BMC Biotechnol. 2016 Nov 21;16(1):82. doi: 10.1186/s12896-016-0312-7.
5
Characterization of a novel thermostable GH45 endoglucanase from Chaetomium thermophilum and its biodegradation of pectin.嗜热毛壳菌新型耐热GH45内切葡聚糖酶的表征及其对果胶的生物降解作用
J Biosci Bioeng. 2017 Sep;124(3):271-276. doi: 10.1016/j.jbiosc.2017.03.017. Epub 2017 May 19.
6
Functional characterization of a bacterial expansin from Bacillus subtilis for enhanced enzymatic hydrolysis of cellulose.来自枯草芽孢杆菌的一种细菌伸展蛋白对纤维素酶促水解增强作用的功能表征。
Biotechnol Bioeng. 2009 Apr 1;102(5):1342-53. doi: 10.1002/bit.22193.
7
Synergism between Clostridium thermocellum cellulases cloned in Escherichia coli.在大肠杆菌中克隆的嗜热栖热放线菌纤维素酶之间的协同作用。
Appl Biochem Biotechnol. 1992 Nov;37(2):201-7. doi: 10.1007/BF02921671.
8
[Genes for cellulose-degradation and their expression conditions in Chaetomium globosum NK102].[球毛壳菌NK102中纤维素降解相关基因及其表达条件]
Wei Sheng Wu Xue Bao. 2013 Apr 4;53(4):354-62.
9
Type-dependent action modes of AA9E and AA9A acting on cellulose and differently pretreated lignocellulosic substrates.AA9E和AA9A作用于纤维素及不同预处理木质纤维素底物的类型依赖性作用模式。
Biotechnol Biofuels. 2017 Feb 22;10:46. doi: 10.1186/s13068-017-0721-4. eCollection 2017.
10
Isolation of cellulolytic mutants of thermotolerant fungus Chaetomium cellulolyticum ATCC 32319.
Acta Microbiol Pol. 1985;34(3-4):255-60.

引用本文的文献

1
Efficient Secretory Production of Lytic Polysaccharide Monooxygenase LPMO10 and Its Application in Plant Biomass Conversion.高效分泌裂解多糖单加氧酶 LPMO10 及其在植物生物质转化中的应用。
Int J Mol Sci. 2023 Jun 3;24(11):9710. doi: 10.3390/ijms24119710.
2
A novel self-purified auxiliary protein enhances the lichenase activity towards lichenan for biomass degradation.一种新型自纯化辅助蛋白提高了对木聚糖的木聚糖酶活性，有助于生物质降解。
Appl Microbiol Biotechnol. 2023 Jul;107(14):4553-4566. doi: 10.1007/s00253-023-12608-y. Epub 2023 Jun 5.
3
Tobacco Plastid Transformation as Production Platform of Lytic Polysaccharide MonoOxygenase Auxiliary Enzymes.

本文引用的文献

1
Cellulolytic activities ofChaetomium globosum on different cellulosic substrates.里氏木霉对不同纤维素底物的纤维素酶活性。
World J Microbiol Biotechnol. 1990 Mar;6(1):23-6. doi: 10.1007/BF01225350.
2
Quantum mechanical calculations suggest that lytic polysaccharide monooxygenases use a copper-oxyl, oxygen-rebound mechanism.量子力学计算表明，溶菌多糖单加氧酶使用铜-氧自由基、氧回弹机制。
Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):149-54. doi: 10.1073/pnas.1316609111. Epub 2013 Dec 16.
3
The carbohydrate-active enzymes database (CAZy) in 2013.
烟草质体转化作为溶细胞多糖单加氧酶辅助酶的生产平台。
Int J Mol Sci. 2022 Dec 24;24(1):309. doi: 10.3390/ijms24010309.
4
The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass.裂解多糖单加氧酶与地衣酶的协同作用及其在二氧化硅纳米球上的共固定化用于地衣生物质的绿色转化
Front Nutr. 2022 Oct 19;9:970540. doi: 10.3389/fnut.2022.970540. eCollection 2022.
5
Evaluation of temperature, pH and nutrient conditions in bacterial growth and extracellular hydrolytic activities of two Alicyclobacillus spp. strains.两种耐酸菌菌株的生长过程中的温度、pH 值和营养条件评估及其胞外水解活性。
Arch Microbiol. 2021 Sep;203(7):4557-4570. doi: 10.1007/s00203-021-02332-4. Epub 2021 Jun 22.
6
Horizontal Gene Transfer and Tandem Duplication Shape the Unique CAZyme Complement of the Mycoparasitic Oomycetes and .水平基因转移和串联重复塑造了寄生卵菌独特的碳水化合物活性酶库
Front Microbiol. 2020 Oct 29;11:581698. doi: 10.3389/fmicb.2020.581698. eCollection 2020.
7
Identification and characterization of a novel AA9-type lytic polysaccharide monooxygenase from a bagasse metagenome.从甘蔗渣宏基因组中鉴定和表征一种新型 AA9 型溶菌多糖单加氧酶。
Appl Microbiol Biotechnol. 2021 Jan;105(1):197-210. doi: 10.1007/s00253-020-11002-2. Epub 2020 Nov 24.
8
Synergistic Action of a Lytic Polysaccharide Monooxygenase and a Cellobiohydrolase from in Cellulose Saccharification under High-Level Substrate Loading.在高负荷底物条件下，溶菌多糖单加氧酶和纤维二糖水解酶协同作用于纤维素糖化。
Appl Environ Microbiol. 2020 Nov 10;86(23). doi: 10.1128/AEM.01769-20.
9
Composition and biosynthetic machinery of the f. sp. conidia cell wall.f. sp. 分生孢子细胞壁的组成与生物合成机制。
Cell Surf. 2019 Aug 14;5:100029. doi: 10.1016/j.tcsw.2019.100029. eCollection 2019 Dec.
10
Functional characterization of cellulose-degrading AA9 lytic polysaccharide monooxygenases and their potential exploitation.纤维素降解 AA9 溶菌多糖单加氧酶的功能表征及其潜在应用。
Appl Microbiol Biotechnol. 2020 Apr;104(8):3229-3243. doi: 10.1007/s00253-020-10467-5. Epub 2020 Feb 19.
2013 版碳水化合物活性酶数据库（CAZy）。
Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5. doi: 10.1093/nar/gkt1178. Epub 2013 Nov 21.
4
Evaluation of bacterial expansin EXLX1 as a cellulase synergist for the saccharification of lignocellulosic Agro-industrial wastes.评价细菌扩展蛋白 EXLX1 作为木质纤维素农业工业废物糖化的纤维素酶增效剂。
PLoS One. 2013 Sep 23;8(9):e75022. doi: 10.1371/journal.pone.0075022. eCollection 2013.
5
Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium.真菌黄孢原毛平革菌 GH61D 溶菌多糖单加氧酶的晶体结构和计算特征。
J Biol Chem. 2013 May 3;288(18):12828-39. doi: 10.1074/jbc.M113.459396. Epub 2013 Mar 22.
6
Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes.扩充 CAZy 数据库中的酶谱，以整合辅助氧化还原酶。
Biotechnol Biofuels. 2013 Mar 21;6(1):41. doi: 10.1186/1754-6834-6-41.
7
Cello-oligosaccharide oxidation reveals differences between two lytic polysaccharide monooxygenases (family GH61) from Podospora anserina.纤寡糖氧化揭示了来自灰盖鬼伞的两种溶菌多糖单加氧酶（GH61 家族）之间的差异。
Appl Environ Microbiol. 2013 Jan;79(2):488-96. doi: 10.1128/AEM.02942-12. Epub 2012 Nov 2.
8
Optimisation of synergistic biomass-degrading enzyme systems for efficient rice straw hydrolysis using an experimental mixture design.采用实验混合设计优化协同生物降解酶系统，以提高水稻秸秆水解效率。
Bioresour Technol. 2012 Sep;119:252-61. doi: 10.1016/j.biortech.2012.05.098. Epub 2012 Jun 1.
9
Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases.真菌多糖单加氧酶底物靶向和催化的结构基础。
Structure. 2012 Jun 6;20(6):1051-61. doi: 10.1016/j.str.2012.04.002. Epub 2012 May 10.
10
Production and effect of aldonic acids during enzymatic hydrolysis of lignocellulose at high dry matter content.在高干物质含量下，木质纤维素酶解过程中醛酸的生成和作用。
Biotechnol Biofuels. 2012 Apr 30;5(1):26. doi: 10.1186/1754-6834-5-26.