de Souza Bernardino Túlio Cezar, Teixeira Antonio Lúcio, Miranda Aline Silva, Guidine Patrícia Maia, Rezende Gustavo, Doretto Maria Carolina, Castro Gabriel Perfeito, Drummond Luciana, Moraes Márcio Flávio Dutra, Tito Pedro Augusto Lopes, de Oliveira Antonio Carlos Pinheiro, Reis Helton José
Department of Pharmacology, Brazil.
Department of Internal Medicine, Brazil.
Neurosci Lett. 2015 Jun 15;597:154-8. doi: 10.1016/j.neulet.2015.04.046. Epub 2015 Apr 30.
Increasing body of evidence suggests that inflammatory and neurotrophic factors might be important for epileptogenesis. Most animal studies demonstrated altered levels of these mediators in drug-induced models of seizures and epilepsy. In the present study, we investigated the production of cytokines and a neurotrophin in the brain of Wistar Audiogenic Rats (WAR), a genetic model of epilepsy, stimulated with high-intensity sound. Four hours after stimulation, animals were decapitated and the hippocampus, inferior colliculus, striatum and cortex were removed for evaluation of the levels of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and brain derived neurotrophic factor (BDNF). All the cytokines and BDNF levels were increased in the cortex. Increased levels of TNF-α and IL-6 were also observed in the striatum. Finally, TNF-α also increased in the inferior colliculus after the seizures induced by high-intensity sound. Although different studies have demonstrated that the levels of cytokines and BDNF increase in animal models of epilepsy induced by chemical stimuli, we provided here evidence that these mediators are also increased in WAR, a genetic model of epilepsy. Thus, the observed increase in these mediators might be involved in the pathophysiology of epilepsy.
越来越多的证据表明，炎症因子和神经营养因子可能在癫痫发生过程中起重要作用。大多数动物研究表明，在药物诱导的癫痫发作和癫痫模型中，这些介质的水平发生了改变。在本研究中，我们调查了高强度声音刺激下，癫痫遗传模型——威斯塔听源性大鼠（WAR）大脑中细胞因子和一种神经营养因子的产生情况。刺激4小时后，将动物断头，取出海马体、下丘、纹状体和皮质，以评估白细胞介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α和脑源性神经营养因子（BDNF）的水平。皮质中所有细胞因子和BDNF水平均升高。纹状体中TNF-α和IL-6水平也升高。最后，高强度声音诱发癫痫发作后，下丘中的TNF-α也升高。尽管不同研究表明，化学刺激诱导的癫痫动物模型中细胞因子和BDNF水平会升高，但我们在此提供证据表明，这些介质在癫痫遗传模型WAR中也会升高。因此，观察到的这些介质水平升高可能参与了癫痫的病理生理过程。
