Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto-SP Brazil.
Department of Morphology, Physiology and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto-SP Brazil.
Neurosci Lett. 2020 Mar 16;721:134830. doi: 10.1016/j.neulet.2020.134830. Epub 2020 Feb 7.
Here, we investigated the participation of pro and anti-inflammatory cytokines in the spread of repeated audiogenic seizures from brainstem auditory structures to limbic areas, including the hippocampus. We used Wistar Audiogenic Rats (WARs) and Wistars submitted to the audiogenic kindling protocol with a loud broad-band noise. We measured pro and anti-inflammatory cytokines and nitrate levels in the hippocampus of stimulated animals. Our results show that all WARs developed audiogenic seizures that evolved to limbic seizures whereas seizure-resistant controls did not present any seizures. However, regardless of seizure severity, we did not observe differences in the pro inflammatory cytokines IL-1β, IL-6, TNF-α and IFN-α or in the anti-inflammatory IL-10 in the hippocampi of audiogenic and resistant animals. We also did not find any differences in nitrate content. Our data indicate that the spread of seizures during the audiogenic kindling is not dependent on hippocampal release of cytokines or oxidative stress, but the severity of brainstem seizures will be higher in animals with higher levels of cytokines and the oxidative stress marker, nitrate.
在这里，我们研究了促炎和抗炎细胞因子在重复听觉性癫痫从脑干听觉结构向包括海马体在内的边缘区域扩散中的作用。我们使用了 Wistar 听觉诱发大鼠（WARs）和接受听觉诱发惊厥方案的 Wistar 大鼠，使用响亮的宽带噪声进行刺激。我们测量了海马体中促炎和抗炎细胞因子以及硝酸盐水平。我们的结果表明，所有 WARs 均发生听觉性癫痫发作，并发展为边缘性癫痫发作，而抗惊厥对照动物则未出现任何癫痫发作。然而，无论癫痫发作的严重程度如何，我们在听觉性和抗性动物的海马体中均未观察到促炎细胞因子 IL-1β、IL-6、TNF-α 和 IFN-α 或抗炎细胞因子 IL-10 的差异。我们也未发现硝酸盐含量的差异。我们的数据表明，听觉性惊厥过程中的癫痫发作扩散并不依赖于海马体释放细胞因子或氧化应激，但具有更高细胞因子和氧化应激标志物硝酸盐水平的动物的脑干癫痫发作严重程度会更高。
