微小RNA-19a通过靶向BTG1调节去势抵抗性前列腺癌细胞的增殖和凋亡。
MicroRNA-19a regulates proliferation and apoptosis of castration-resistant prostate cancer cells by targeting BTG1.
作者信息
Lu Kai, Liu Chunhui, Tao Tao, Zhang Xiaowen, Zhang Lei, Sun Chao, Wang Yiduo, Chen Shuqiu, Xu Bin, Chen Ming
机构信息
Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China.
Department of Medicine, Southeast University, Nanjing, China.
出版信息
FEBS Lett. 2015 Jun 4;589(13):1485-90. doi: 10.1016/j.febslet.2015.04.037. Epub 2015 Apr 30.
MicroRNAs (miRNAs) play a significant role in tumor development. Recent studies indicate that miRNAs are implicated in prostate cancer (PCa). In this study, we found that miR-19a expression was significantly increased in castration-resistant prostate cancer (CRPC) tissues compared with androgen-dependent prostate cancer (ADPC) tissues. We found that inhibiting the overexpression of miR-19a in CRPC cells suppressed proliferation and increased apoptosis. Additionally, we found that miR-19a repressed BTG1 expression by binding to its 3'-untranslated region. The overexpression of BTG1 in CRPC cells significantly suppressed proliferation and increased apoptosis. We conclude that miR-19a regulates proliferation and apoptosis of CRPC cells by directly targeting the tumor suppressor gene BTG1.
微小RNA（miRNA）在肿瘤发展中发挥着重要作用。最近的研究表明，miRNA与前列腺癌（PCa）有关。在本研究中，我们发现与雄激素依赖性前列腺癌（ADPC）组织相比，去势抵抗性前列腺癌（CRPC）组织中miR-19a的表达显著增加。我们发现抑制CRPC细胞中miR-19a的过表达可抑制增殖并增加细胞凋亡。此外，我们发现miR-19a通过与BTG1的3'-非翻译区结合来抑制其表达。CRPC细胞中BTG1的过表达显著抑制了增殖并增加了细胞凋亡。我们得出结论，miR-19a通过直接靶向肿瘤抑制基因BTG1来调节CRPC细胞的增殖和凋亡。
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