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乳酸菌抗菌和抗真菌活性的高通量筛选试验

High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus species.

作者信息

Inglin Raffael C, Stevens Marc J A, Meile Lukas, Lacroix Christophe, Meile Leo

机构信息

Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.

Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.

出版信息

J Microbiol Methods. 2015 Jul;114:26-9. doi: 10.1016/j.mimet.2015.04.011. Epub 2015 Apr 30.

DOI:10.1016/j.mimet.2015.04.011
PMID:25937247
Abstract

We describe high-throughput screening techniques to rapidly detect either antimicrobial activity, using an agar-well diffusion assay in microtiter plates, or antifungal activity using an agar-spot assay in 24-well plates. 504 Lactobacillus isolates were screened with minimal laboratory equipment and screening rates of 2000-5000 individual antimicrobial interactions.

摘要

我们描述了高通量筛选技术，以快速检测抗菌活性（使用微量滴定板中的琼脂孔扩散试验）或抗真菌活性（使用24孔板中的琼脂斑点试验）。使用最少的实验室设备对504株乳酸杆菌分离株进行了筛选，筛选率为2000-5000个个体抗菌相互作用。

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