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产胞外多糖的EPS DA - BACS的潜在益生菌特性及其胞外多糖的益生元活性

Potential Probiotic Properties of Exopolysaccharide-Producing EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide.

作者信息

Lee Min-Gyu, Joeng Huijin, Shin Jaein, Kim Suin, Lee Chaeeun, Song Youngbo, Lee Byung-Hoo, Park Hyoung-Geun, Lee Tae-Ho, Jiang Hai-Hua, Han Young-Sun, Lee Bong-Gyeong, Lee Ho-Jin, Park Min-Ju, Jun Yun-Ju, Park Young-Seo

机构信息

Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of Korea.

Research Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin 17073, Republic of Korea.

出版信息

Microorganisms. 2022 Dec 8;10(12):2431. doi: 10.3390/microorganisms10122431.

DOI:10.3390/microorganisms10122431
PMID:36557684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9787920/
Abstract

Exopolysaccharide (EPS)-producing EPS DA-BACS was isolated from healthy human feces and its probiotic properties, as well as the structure and prebiotic activity of the EPS from this strain were examined. EPS from EPS DA-BACS had a ropy phenotype, which is known to have potential health benefits and is identified as loosely cell-bounded glucomannan-type EPS with a molecular size of 3.7 × 10 Da. EPS promoted the acid tolerance of EPS DA-BACS and provided cells with tolerance to gastrointestinal stress. The purified EPS showed growth inhibitory activity against EPS DA-BACS cells completely inhibited the growth of , , and , as well as showed high growth inhibitory activity against and . Treatment of lipopolysaccharide-stimulated RAW 264.7 cells with heat-killed EPS DA-BACS cells led to a decrease in the production of nitric oxide, indicating the anti-inflammatory activity of EPS DA-BACS. Purified EPS promoted the growth of , and which showed high prebiotic activity. EPS DA-BACS harbors no antibiotic resistance genes or virulence factors. Therefore, EPS DA-BACS exhibits anti-inflammatory and antimicrobial activities with high gut adhesion ability and gastrointestinal tolerance and can be used as a potential probiotic.

摘要

从健康人粪便中分离出产胞外多糖（EPS）的EPS DA - BACS，并对其益生菌特性以及该菌株EPS的结构和益生元活性进行了研究。来自EPS DA - BACS的EPS具有粘稠表型，已知具有潜在的健康益处，被鉴定为分子大小为3.7×10 Da的松散细胞结合型葡甘露聚糖型EPS。EPS促进了EPS DA - BACS的耐酸性，并为细胞提供了对胃肠道应激的耐受性。纯化的EPS对EPS DA - BACS细胞表现出生长抑制活性，完全抑制了[具体菌株1]、[具体菌株2]和[具体菌株3]的生长，同时对[具体菌株4]和[具体菌株5]也表现出高生长抑制活性。用热灭活的EPS DA - BACS细胞处理脂多糖刺激的RAW 264.7细胞导致一氧化氮产生减少，表明EPS DA - BACS具有抗炎活性。纯化的EPS促进了[具体菌株6]、[具体菌株7]和[具体菌株8]的生长，显示出高益生元活性。EPS DA - BACS不携带抗生素抗性基因或毒力因子。因此，EPS DA - BACS具有抗炎和抗菌活性，具有高肠道粘附能力和胃肠道耐受性，可作为潜在的益生菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/e6a139a06fdb/microorganisms-10-02431-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/4c484ddc3cea/microorganisms-10-02431-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/fd373de03536/microorganisms-10-02431-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/273081204603/microorganisms-10-02431-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/6078e65d6071/microorganisms-10-02431-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/c82e1d2fa82f/microorganisms-10-02431-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/55553c73b98c/microorganisms-10-02431-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/27fa610fd0dc/microorganisms-10-02431-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/e6a139a06fdb/microorganisms-10-02431-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/3a3205b3a0e6/microorganisms-10-02431-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/4c484ddc3cea/microorganisms-10-02431-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/ea9d4bee903b/microorganisms-10-02431-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/a8ae01e7bc22/microorganisms-10-02431-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/fd373de03536/microorganisms-10-02431-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/273081204603/microorganisms-10-02431-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/6078e65d6071/microorganisms-10-02431-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/c82e1d2fa82f/microorganisms-10-02431-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/55553c73b98c/microorganisms-10-02431-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/27fa610fd0dc/microorganisms-10-02431-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b96/9787920/e6a139a06fdb/microorganisms-10-02431-g011.jpg

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