一种利用淡水轮虫（萼花臂尾轮虫）饲养斑马鱼的新方法。
A Novel Method for Rearing Zebrafish by Using Freshwater Rotifers (Brachionus calyciflorus).
作者信息
Aoyama Yuta, Moriya Natsumi, Tanaka Shingo, Taniguchi Tomoko, Hosokawa Hiroshi, Maegawa Shingo
机构信息
1 Division of Cognitive and Information Sciences, Faculty of Integrated Human Studies, Kyoto University , Kyoto, Japan .
2 Department of Intelligence Science and Technology, Graduate School of Informatics, Kyoto University , Kyoto, Japan .
出版信息
Zebrafish. 2015 Aug;12(4):288-95. doi: 10.1089/zeb.2014.1032. Epub 2015 May 4.
The zebrafish (Danio rerio) has become a powerful model organism for studying developmental processes and genetic diseases. However, there remain several problems in previous rearing methods. In this study, we demonstrate a novel method for rearing zebrafish larvae by using a new first food, freshwater rotifers (Brachionus calyciflorus). Feeding experiments indicated that freshwater rotifers are suitable as the first food for newly hatched larval fish. In addition, we revisited and improved a feeding schedule from 5 to 40 days postfertilization. Our feeding method using freshwater rotifers accelerated larval growth. At 49 dpf, one pair out of 10 pairs successfully produced six fertilized eggs. At 56, 63, and 71 dpf, 6 out of the 10 pairs constantly produced normal embryos. Our method will improve the husbandry of the zebrafish.
斑马鱼（Danio rerio）已成为研究发育过程和遗传疾病的强大模式生物。然而，以前的饲养方法仍存在一些问题。在本研究中，我们展示了一种使用新型开口饲料淡水轮虫（萼花臂尾轮虫）饲养斑马鱼幼体的新方法。喂养实验表明，淡水轮虫适合作为新孵化幼鱼的开口饲料。此外，我们重新审视并改进了受精后5至40天的喂养时间表。我们使用淡水轮虫的喂养方法加速了幼体生长。在受精后49天，10对中有1对成功产出了6枚受精卵。在受精后56、63和71天，10对中有6对持续产出正常胚胎。我们的方法将改善斑马鱼的饲养管理。
Zotero 插件