Paltoglou George, Fatouros Ioannis G, Valsamakis George, Schoina Maria, Avloniti Alexandra, Chatzinikolaou Athanasios, Kambas Antonis, Draganidis Dimitris, Mantzou Aimilia, Papagianni Maria, Kanaka-Gantenbein Christina, Chrousos George P, Mastorakos George
1] Endocrine Unit, Aretaieion Hospital, University of Athens Medical School, Athens, Greece [2] First Department of Pediatrics, "Aghia Sofia" Children's Hospital, University of Athens Medical School, Athens, Greece.
School of Physical Education and Sports Sciences, Democritus University of Thrace, Komotini, Greece.
Pediatr Res. 2015 Aug;78(2):158-64. doi: 10.1038/pr.2015.85. Epub 2015 May 4.
Oxidative stress is associated with obesity while the evidence for the role of GH in pro- and antioxidation is inconclusive. This study investigates the relationships between growth hormone (GH), pro- and antioxidation in relation to obesity and puberty before and after an acute bout of exercise.
In this case-control study, 76 healthy normal-weight and obese, prepubertal and pubertal boys underwent a blood sampling before and immediately after an aerobic exercise bout until exhaustion at 70% maximal oxygen consumption. Markers of prooxidation (thiobarbituric acid reactive substances (TBARS) and protein carbonyls (PCs)) and antioxidation (glutathione (GSH), oxidized glutathione disulfide (GSSG), GSH/GSSG ratio, glutathione peroxidase (GPX), catalase, and total antioxidant capacity (TAC)) and hormones (GH, insulin-like growth factor (IGF)-1, IGF-BP-3, luteinizing hormone, follicle-stimulating hormone, and testosterone) were measured.
Baseline and postexercise TBARS and PCs were greater, while baseline GSH, GSH/GSSG ratio, GPX, and TAC were lower in obese than that in normal-weight participants. In all participants, waist was the best negative and positive predictor for postexercise GPX and TBARS, respectively. Baseline TAC was greater in pubertal than that in pre-pubertal participants. In all participants, baseline GH was the best negative predictor for postexercise PCs. Significant positive linear correlation exists between the exercise-associated GH, and GSSG increases in pubertal normal-weight boys.
Higher prooxidation and lower antioxidation were observed in obese boys, while antioxidation improves with puberty and postexercise, paralleling GH accentuated secretion.
氧化应激与肥胖相关，而生长激素（GH）在促氧化和抗氧化作用方面的证据尚无定论。本研究调查了急性运动前后，生长激素（GH）、促氧化和抗氧化与肥胖及青春期之间的关系。
在这项病例对照研究中，76名健康的正常体重和肥胖的青春期前及青春期男孩在有氧运动直至耗竭（最大耗氧量的70%）之前和之后立即进行了血液采样。测量了促氧化指标（硫代巴比妥酸反应性物质（TBARS）和蛋白质羰基（PCs））、抗氧化指标（谷胱甘肽（GSH）、氧化型谷胱甘肽二硫化物（GSSG）、GSH/GSSG比值、谷胱甘肽过氧化物酶（GPX）、过氧化氢酶和总抗氧化能力（TAC））以及激素（GH、胰岛素样生长因子（IGF）-1、IGF结合蛋白-3、黄体生成素、卵泡刺激素和睾酮）。
肥胖参与者的基线和运动后TBARS及PCs水平更高，而基线GSH、GSH/GSSG比值、GPX和TAC水平低于正常体重参与者。在所有参与者中，腰围分别是运动后GPX和TBARS的最佳负向和正向预测指标。青春期参与者的基线TAC高于青春期前参与者。在所有参与者中，基线GH是运动后PCs的最佳负向预测指标。青春期正常体重男孩中，运动相关的GH增加与GSSG增加之间存在显著的正线性相关性。
肥胖男孩中观察到更高的促氧化和更低的抗氧化水平，而抗氧化能力随着青春期和运动后而改善，与GH分泌增加平行。
