Pei Ming, Li Jingting, McConda David B, Wen Sijin, Clovis Nina B, Danley Suzanne S
Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, Morgantown, WV 26506-9196, USA; Division of Exercise Physiology, West Virginia University, Morgantown, WV 26506-9227, USA.
Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, Morgantown, WV 26506-9196, USA; Division of Exercise Physiology, West Virginia University, Morgantown, WV 26506-9227, USA.
Bone. 2015 Sep;78:1-10. doi: 10.1016/j.bone.2015.04.040. Epub 2015 May 1.
Repairing large bone defects presents a significant challenge, especially in those people who have a limited regenerative capacity such as in osteoporotic (OP) patients. The aim of this study was to compare adipose stem cells (ASCs) from both normal (NORM) and ovariectomized (OVX) rats in osteogenic potential using both in vitro and in vivo models. After successful establishment of a rat OP model, we found that ASCs from OVX rats exhibited a comparable proliferation capacity to those from NORM rats but had significantly higher adipogenic and relatively lower osteogenic potential. Thirty-two weeks post-implantation with poly(lactic-co-glycolic acid) (PLGA) alone or PLGA seeded with osteogenic-induced ASCs for critical-size calvarial defects, the data from Herovici's collagen staining and micro-computed tomography suggested that the implantation of ASC-PLGA constructs exhibited a higher bone volume density compared to the PLGA alone group, especially in the NORM rat group. Intriguingly, the defects from OVX rats exhibited a higher bone volume density compared to NORM rats, especially for implantation of the PLGA alone group. Our results indicated that ASC based tissue constructs are more beneficial for the repair of calvarial defects in NORM rats while implantation of PLGA scaffold contributed to defect regeneration in OVX rats.
修复大的骨缺损是一项重大挑战，尤其是对于那些再生能力有限的人群，如骨质疏松（OP）患者。本研究的目的是使用体外和体内模型比较正常（NORM）大鼠和去卵巢（OVX）大鼠的脂肪干细胞（ASC）的成骨潜力。成功建立大鼠OP模型后，我们发现来自OVX大鼠的ASC与来自NORM大鼠的ASC具有相当的增殖能力，但具有明显更高的成脂潜力和相对较低的成骨潜力。单独植入聚乳酸-乙醇酸共聚物（PLGA）或植入接种有成骨诱导ASC的PLGA 32周后，用于关键尺寸颅骨缺损，来自赫罗维奇胶原染色和微型计算机断层扫描的数据表明，与单独的PLGA组相比，ASC-PLGA构建体的植入表现出更高的骨体积密度，尤其是在NORM大鼠组。有趣的是，与NORM大鼠相比，OVX大鼠的缺损表现出更高的骨体积密度，尤其是单独植入PLGA组。我们的结果表明，基于ASC的组织构建体对NORM大鼠颅骨缺损的修复更有益，而PLGA支架的植入有助于OVX大鼠的缺损再生。
