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The efficiency of 1 M NH4Cl and 2 M NaCl for the isolation of pathogenic Nocardia from soil.

作者信息

van Gelderen de Komaid A, Durán E L

机构信息

Instituto de Microbiología Dr. Luis C. Verna, Facultad de Bioquimica, Universidad Nacional de Tucumán, República Argentina.

出版信息

Mycopathologia. 1989 Nov;108(2):117-23. doi: 10.1007/BF00436062.

DOI:10.1007/BF00436062
PMID:2594047
Abstract

The present paper reports an improvement to the classical method of the paraffin bait, by the usage of 1 M NH4Cl or 2 M NaCl to eliminate contaminant microflora of soil. The purpose is to introduce a change in the paraffin bait method in order to reduce time required to isolate pathogenic strains of Nocardia from their natural sources. For this study three main criteria were used: a) Determination of the inhibitory effect of different concentrations of salts on soil microflora; b) The tolerance of Nocardia brasiliensis, Nocardia asteroides and Nocardia caviae (Nocardia otitidis caviarum) strains to these chemical inhibitors; c) Determination of the efficiency of salts in the isolation of Nocardia from soil when strains are grown on paraffin baits.

摘要

相似文献

1
The efficiency of 1 M NH4Cl and 2 M NaCl for the isolation of pathogenic Nocardia from soil.
Mycopathologia. 1989 Nov;108(2):117-23. doi: 10.1007/BF00436062.
2
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引用本文的文献

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Comparison of three phenotypic and deoxyribonucleic acid extraction methods for isolation and Identification of Nocardia spp.三种用于分离和鉴定诺卡氏菌属的表型和脱氧核糖核酸提取方法的比较
Adv Biomed Res. 2014 Jul 31;3:151. doi: 10.4103/2277-9175.137839. eCollection 2014.
2
Acid and alkali as aids in differentiation of pathogenic Nocardia and for their isolation from clinical specimens.
Mycopathologia. 1991 Mar;113(3):133-7. doi: 10.1007/BF00436114.

本文引用的文献

1
Isolation of Nocardia caviae from Soil and Its Pathogenicity for Laboratory Animals.从土壤中分离出豚鼠诺卡菌及其对实验动物的致病性。
J Bacteriol. 1965 Sep;90(3):822-3. doi: 10.1128/jb.90.3.822-823.1965.
2
[Isolation of Nocardia brasiliensis and asteroides from soils].[从土壤中分离巴西诺卡菌和星状诺卡菌]
Rev Inst Salubr Enferm Trop. 1960 Sep;20:147-51.
3
Nocardia in soils of southeastern Spain: abundance, distribution, and chemical characterisation.西班牙东南部土壤中的诺卡氏菌：丰度、分布及化学特征
Can J Microbiol. 1984 Aug;30(8):1088-92. doi: 10.1139/m84-169.
4
A survey of Nocardia asteroides, N. caviae and N. brasiliensis occuring in soil in India.对印度土壤中出现的星状诺卡菌、豚鼠诺卡菌和巴西诺卡菌的一项调查。
Sabouraudia. 1968 Jun;6(3):260-6.
5
Application of paraffin bait technique to the isolation of Nocardia asteroides from clinical specimens.石蜡诱饵技术在从临床标本中分离星形诺卡菌中的应用。
Appl Microbiol. 1969 Oct;18(4):686-7. doi: 10.1128/am.18.4.686-687.1969.
6
Observations on paraffin baiting as a laboratory diagnostic procedure in nocardiosis.关于石蜡诱饵法作为诺卡菌病实验室诊断方法的观察
Mycopathol Mycol Appl. 1973 Nov 15;51(2):147-57. doi: 10.1007/BF02141106.
7
Comparison of paraffin baiting and conventional culture techniques for isolation of Nocardia asteroides from sputum.痰液中分离星形诺卡菌的石蜡诱饵法与传统培养技术的比较
J Clin Microbiol. 1987 Jan;25(1):176-7. doi: 10.1128/jcm.25.1.176-177.1987.
8
Natural occurrence of Nocardia in soil of Tucumán: physiological characteristics.图克曼土壤中诺卡氏菌的自然存在：生理特征。
Mycopathologia. 1987 Jul;99(1):15-9. doi: 10.1007/BF00436675.
9
Physiological and pathogenic characteristics of Nocardia brasiliensis isolated from human mycetomas.从人类足菌肿中分离出的巴西奴卡菌的生理和致病特征。
Mycopathologia. 1989 Feb;105(2):111-6. doi: 10.1007/BF00444033.