Kim Ha-Jung, Ahrens Kim, Park Hee-Myung, Marsella Rosanna
Department of Dermatology, Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL, 32615, USA.
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, #1 Whayang-dong, Kwangjin-gu, Seoul, South Korea.
Vet Dermatol. 2015 Jun;26(3):180-5, e36-7. doi: 10.1111/vde.12203. Epub 2015 May 4.
Protease-activated receptor (PAR)-2 plays a crucial role in inflammation and the skin barrier. Protease-activated receptor-2 is activated by proteolytic enzymes of allergens and stimulates thymic stromal lymphopoietin (TSLP), promoting T-helper 2 cytokines. In humans with atopic dermatitis (AD), increased expression of PAR-2 and TSLP has been reported.
HYPOTHESIS/OBJECTIVES: To compare the pattern of staining of PAR-2 and TSLP between normal and atopic beagle dogs. The hypothesis tested was that increased expression is present in atopic dog skin compared with healthy control skin.
Eight atopic and five normal dogs were challenged for 3 days with house dust mites.
Skin biopsies were taken to measure the intensity, distribution, integrity and cell staining pattern on days 0, 3 and 10, both objectively and subjectively. Clinical signs were scored and compared between groups.
Atopic dogs showed a significant increase in clinical scores on days 3 (peak of challenge) and 10 (resolution) and a significant condensed staining pattern for TSLP in the stratum basale at all times in comparison to normal dogs. They showed a significant patchy pattern for PAR-2 on days 0 and 3 and for TSLP at all times compared with normal dogs. The intensity itself was not significantly increased in atopic dogs compared with normal animals for both PAR-2 and TSLP.
These preliminary findings do not confirm a difference in the amount of expression but rather in its pattern. Studies using PAR-2 or TSLP inhibitors could shed light on their clinical relevance.
蛋白酶激活受体（PAR）-2在炎症和皮肤屏障中起关键作用。蛋白酶激活受体-2被过敏原的蛋白水解酶激活，并刺激胸腺基质淋巴细胞生成素（TSLP），促进辅助性T细胞2细胞因子的产生。在特应性皮炎（AD）患者中，已报道PAR-2和TSLP的表达增加。
假设/目的：比较正常和特应性比格犬之间PAR-2和TSLP的染色模式。所检验的假设是，与健康对照皮肤相比，特应性犬皮肤中存在表达增加。
8只特应性犬和5只正常犬用屋尘螨攻击3天。
在第0、3和10天取皮肤活检组织，客观和主观地测量其强度、分布、完整性和细胞染色模式。对临床症状进行评分并在组间进行比较。
与正常犬相比，特应性犬在第3天（攻击高峰）和第10天（消退期）的临床评分显著增加，并且在所有时间点，基底细胞层中TSLP的染色模式显著浓缩。与正常犬相比，它们在第0天和第3天PAR-2呈现显著的斑片状模式，并且在所有时间点TSLP均呈现显著的斑片状模式。与正常动物相比，特应性犬中PAR-2和TSLP的强度本身没有显著增加。
这些初步发现并未证实表达量存在差异，而是证实了表达模式存在差异。使用PAR-2或TSLP抑制剂的研究可能会揭示它们的临床相关性。
