Klukowska-Rötzler Jolanta, Chervet Ludovic, Müller Eliane J, Roosje Petra, Marti Eliane, Janda Jozef
Department of Clinical Research-VPH, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, CH-3012 Bern, Switzerland.
Vet Dermatol. 2013 Feb;24(1):54-9.e13-4. doi: 10.1111/j.1365-3164.2012.01096.x.
In humans, thymic stromal lymphopoietin (TSLP) plays a central role in the development of allergic inflammation, such as atopic dermatitis (AD), but it is unknown whether it is involved in the pathogenesis of canine AD (CAD).
HYPOTHESIS/OBJECTIVES: Our aim was to characterize canine TSLP and to assess its expression in CAD.
Canine TSLP was identified based on sequence homology with human TSLP and the complementary DNA (cDNA) cloned by RT-PCR. Real-time quantitative RT-PCR was established to assess the expression of canine TSLP in cultured canine keratinocytes and in skin biopsy specimens from lesional and nonlesional skin of 12 dogs with CAD and eight healthy control dogs.
Partial canine TSLP cDNA was cloned and characterized. It contained four exons that shared 70 and 73% nucleotide identity with human and equine TSLP, respectively, encoding the signal peptide and full-length secreted protein. We found significantly increased TSLP expression in lesional and nonlesional skin of dogs with CAD compared with healthy control dogs (P < 0.05), whereas no difference was measured between lesional and nonlesional samples. In cultured primary canine keratinocytes, we found increased TSLP expression after stimulation with house dust mite allergen extract or Toll-like receptor ligands lipopolysaccharide and poly I:C.
Increased TSLP expression in the skin of dogs with CAD supports an involvement of TSLP in the pathogenesis of CAD similar to that in humans. Further studies should elucidate the function and therapeutic potential of TSLP in CAD.
在人类中，胸腺基质淋巴细胞生成素（TSLP）在过敏性炎症（如特应性皮炎，AD）的发展中起核心作用，但尚不清楚它是否参与犬类特应性皮炎（CAD）的发病机制。
假设/目的：我们的目的是对犬类TSLP进行特性分析，并评估其在CAD中的表达。
基于与人类TSLP的序列同源性以及通过逆转录聚合酶链反应（RT-PCR）克隆的互补DNA（cDNA）来鉴定犬类TSLP。建立实时定量RT-PCR以评估犬类TSLP在培养的犬角质形成细胞以及来自12只患有CAD的犬和8只健康对照犬的病变皮肤和非病变皮肤的皮肤活检标本中的表达。
克隆并鉴定了部分犬类TSLP cDNA。它包含四个外显子，分别与人类和马的TSLP共享70%和73%的核苷酸同一性，编码信号肽和全长分泌蛋白。我们发现，与健康对照犬相比，患有CAD的犬的病变皮肤和非病变皮肤中TSLP表达显著增加（P < 0.05），而病变样本和非病变样本之间未检测到差异。在培养的原代犬角质形成细胞中，我们发现用屋尘螨变应原提取物或Toll样受体配体脂多糖和聚肌苷酸胞苷酸刺激后TSLP表达增加。
患有CAD的犬皮肤中TSLP表达增加支持TSLP参与CAD的发病机制，这与人类相似。进一步的研究应阐明TSLP在CAD中的功能和治疗潜力。
