Ossiboff Robert J, Newton Alisa L, Seimon Tracie A, Moore Robert P, McAloose Denise
Wildlife Conservation Society, Zoological Health Program, Bronx, NY
Wildlife Conservation Society, Zoological Health Program, Bronx, NY.
J Vet Diagn Invest. 2015 May;27(3):392-5. doi: 10.1177/1040638715584793. Epub 2015 May 5.
A captive, juvenile, female northern map turtle (Graptemys geographica) was found dead following a brief period of weakness and nasal discharge. Postmortem examination identified pneumonia with necrosis and numerous epithelial, intranuclear viral inclusion bodies, consistent with herpesviral pneumonia. Similar intranuclear inclusions were also associated with foci of hepatocellular and splenic necrosis. Polymerase chain reaction (PCR) screening of fresh, frozen liver for the herpesviral DNA-dependent DNA polymerase gene yielded an amplicon with 99.2% similarity to recently described emydid herpesvirus 1 (EmyHV-1). Molecular screening of turtles housed in enclosures that shared a common circulation system with the affected map turtle identified 4 asymptomatic, EmyHV-1 PCR-positive painted turtles (Chrysemys picta) and 1 asymptomatic northern map turtle. Herpesvirus transmission between painted and map turtles has been previously suggested, and our report provides the molecular characterization of a herpesvirus in asymptomatic painted turtles that can cause fatal herpesvirus-associated disease in northern map turtles.
一只圈养的幼年雌性北部地图龟（Graptemys geographica）在出现短暂的虚弱和鼻腔分泌物后被发现死亡。尸检发现有坏死性肺炎以及大量上皮细胞核内病毒包涵体，符合疱疹病毒性肺炎。类似的核内包涵体也与肝细胞和脾脏坏死灶有关。对新鲜冷冻肝脏进行聚合酶链反应（PCR）筛查疱疹病毒DNA依赖性DNA聚合酶基因，得到一个与最近描述的水龟疱疹病毒1（EmyHV-1）相似度为99.2%的扩增子。对与受影响的地图龟共用一个循环系统的围栏中的海龟进行分子筛查，发现4只无症状的EmyHV-1 PCR阳性彩龟（Chrysemys picta）和1只无症状的北部地图龟。此前曾有人提出疱疹病毒在彩龟和地图龟之间传播，我们的报告提供了无症状彩龟中一种疱疹病毒的分子特征，这种病毒可导致北部地图龟患上致命的疱疹病毒相关疾病。
