Wang Jianfeng, Lin Kan, Zheng Wei, Ho Khek Yu, Teh Ming, Yeoh Khay Guan, Huang Zhiwei
Optical Bioimaging Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore, 117576, Singapore.
Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, Singapore, 119260, Singapore.
Anal Bioanal Chem. 2015 Nov;407(27):8303-10. doi: 10.1007/s00216-015-8727-x. Epub 2015 May 6.
This study aims to compare the diagnostic performance of the two different endoscope-based fiber-optic Raman probe designs (i.e., bevelled and volume Raman probes) for real-time, in vivo detection of gastric dysplasia at endoscopy. To conduct the clinical comparison, a total of 1,050 in vivo tissue Raman spectra (normal: n = 864; dysplasia: n = 186) were acquired from 66 gastric patients (normal: n = 48; dysplasia: n = 18) by using bevelled Raman probe, while a total of 1,913 in vivo tissue Raman spectra (normal: n = 1,786; dysplasia: n = 127) were acquired from 98 gastric patients (normal: n = 87; dysplasia: n = 11) by using volume Raman probe. The bevelled Raman probe provides approximately twofold improvements in tissue Raman-to-autofluorescence intensity ratios as compared to the use of volume Raman probe. Partial least squares discriminant analysis together with leave-one patient-out cross-validation on in vivo tissue Raman spectra acquired yields a diagnostic accuracy of 93.0 % (sensitivity of 92.5 %; specificity of 93.1 %) for differentiating gastric dysplasia from normal gastric tissue by using the bevelled fiber-optic Raman probe, which is superior to the diagnostic performance (accuracy of 88.4 %; sensitivity of 85.8 %; specificity of 88.6 %) by using the volume Raman probe. This work demonstrates that the Raman spectroscopic technique coupled with bevelled fiber-optic Raman probe has great potential to enhance in vivo diagnosis of gastric precancer and early cancer at endoscopy. Graphical Abstract Comparison of in vivo gastric tissue Raman spectra acquired by using bevelled and volume fiber-optic Raman probes.
本研究旨在比较两种不同的基于内窥镜的光纤拉曼探头设计（即斜面拉曼探头和体拉曼探头）在内镜检查时对胃发育异常进行实时、体内检测的诊断性能。为进行临床比较，使用斜面拉曼探头从66例胃部患者（正常：n = 48；发育异常：n = 18）中获取了总共1050条体内组织拉曼光谱（正常：n = 864；发育异常：n = 186），而使用体拉曼探头从98例胃部患者（正常：n = 87；发育异常：n = 11）中获取了总共1913条体内组织拉曼光谱（正常：n = 1786；发育异常：n = 127）。与使用体拉曼探头相比，斜面拉曼探头在组织拉曼与自发荧光强度比方面提供了约两倍的改善。对获取的体内组织拉曼光谱进行偏最小二乘判别分析并结合留一患者交叉验证，使用斜面光纤拉曼探头区分胃发育异常与正常胃组织的诊断准确率为93.0%（灵敏度为92.5%；特异性为93.1%），优于使用体拉曼探头的诊断性能（准确率为88.4%；灵敏度为85.8%；特异性为88.6%）。这项工作表明，拉曼光谱技术与斜面光纤拉曼探头相结合在增强内窥镜检查时对胃癌前病变和早期癌症的体内诊断方面具有巨大潜力。图形摘要 使用斜面和体光纤拉曼探头获取的体内胃组织拉曼光谱比较。
